HeLa culture, transfection, and labeling of Halo-fusion proteins
By onProtocol for HeLa culture, transfection, and labelling of Halo-fusion proteins.
Single-cell transcriptomic and proteomic analysis of Parkinson’s disease Brains
By onThis article established a single-nucleus transcriptomic profile of the prefrontal cortex from postmortem human brains of six patients with PD and six age-matched controls.
Synaptic Location Is a Determinant of the Detrimental Effects of α-Synuclein Pathology to Glutamatergic Transmission in the Basolateral Amygdala
By onStudy explores α-synuclein's impact on amygdala in Parkinson's. Aggregation affects cortico-BLA transmission, linked to psychiatric deficits in PD.
Isolation of NeuN+ cells from brain tissue (for CUT and RUN)
By onThis protocol describes the steps to isolate NeuN+ cells from brain tissue in preparation for CUT and RUN.
Essential surgeries for the electrophysiological recording from a behaving non-human primate brain 2 (Craniotomy)
By onProtocol for performing a craniotomy in a recording chamber on a cranial implant in nonhuman primates.
Data set of the manuscript “Neuronal hyperactivity-induced oxidant stress promotes in vivo a-synuclein brain spreading”
By onData set of the manuscript "Neuronal hyperactivity-induced oxidant stress promotes in vivo a-synuclein brain spreading."
pCAG-GST-ATG101-W110D/F112D
By onPlasmid: Expresses human ATG101 HORMA W110D/F112D mutant in mammalian cells.
CURTAIN—A unique web-based tool for exploration and sharing of MS-based proteomics data
By onLinks can also be reported in publications allowing readers to further survey the reported data. The authors discuss benefits for the research community of publishing proteomic data containing a shareable web-link.
Cx3cr1-cre;Ercc1-/fl mice
By onMicroglia may experience higher levels of DNA damage and potential senescence due to internal factors.
Assay for Dual Rab GTPase binding to the LRRK2 Armadillo Domain
By onThe LRRK2 Armadillo domain contains multiple Rab GTPase binding sites. This assay shows that the sites can be occupied simultaneously.
fMRI tasks to explore emotion-motor interactions
By onCode for fMRI tasks designed to explore emotion-motor interactions—Active Escape and Approach-Avoidance tasks in the Socal Kinesia and Incentivization for Parkinson's Disease (SKIP) dataset.
Passaging of feeder-free hPSCs
By onThis protocol describes the process of passaging human pluripotent stem cells (hPSCs) for feeder-free culturing of hPSCs using Accutase or ReLeSF.
Evaluating endocytic rate in cells
By onAssess endocytic rate in cells using tagged transferrin (Alexa647) and flow cytometry readout.
H9 ES AAVS1-NGN2 TEX264-/-; PiggyBac-Keima-REEP5
By onES cells modified with CRISPR/Cas9 to lack TEX264, express Keima-REEP5 ER-phagy flux reporter, NEUROG2, and mKeima fluorescent protein. Derived from human embryonic stem cells at the blastocyst stage.
H9 ES AAVS1-NGN2 FLAG-EEA1::TMEM192-HA
By onES cells were modified to produce iNeurons expressing FLAG-tagged EEA1 and TMEM192-HA. NEUROG2 construct was inserted into AAVS1 locus using CRISPR/Cas9. EEA1 was N-terminally tagged with a 3xFLAG tag.
