Standard Operating Procedure: Mouse transcardiac perfusion protocol
By onMouse transcardiac perfusion protocol and storage.
Acute striatal or midbrain fiber photometry in head-fixed mice
By onWhile this protocol focuses recordings from striatum with GCaMP, it can be easily modified to record from other brain regions and with other fluorescent reporters.
Transcriptional analysis of peripheral memory T cells reveals Parkinson’s disease-specific gene signatures–Gene Validation FCS
By onFCS files corresponding to the gene validation experiment described in "Transcriptional analysis of peripheral memory T cells reveals Parkinson’s disease-specific gene signatures"
pAC150-Keima-REEP5
By onSummary: Piggybac vector used to express Keima-REEP5 autophagy flux reporter, a tool to monitor autophagy flux in cells by measuring pH changes in autophagosomes and lysosomes.
Temporal landscape of mitochondrial proteostasis governed by the UPRmt
By onThe authors developed a functional proteomics framework, termed MitoPQ (Mitochondrial Proteostasis Quantification), to dissect the UPRmts role in maintaining proteostasis during stress.
Immunofluorescence staining for postmortem mouse brain tissue
By onThis is a basic protocol for staining mouse brain tissues using immunofluorescence and immunohistochemistry techniques.
Preparation of mouse embryonic fibroblast (MEF) feeder plates for hPSC cultures
By onThis protocol describes the preparation of mouse embryonic fibroblasts (MEFs) as feeder cells for human pluripotent stem cell (hPSC) culture.
CRISPR tagging of the EEA1 gene in H9 ES cells for Endo-IP
By onProtocol for tagging EEA1 gene in human H9 ES cells with a 3X flag using CAS9 and oligonucleotide template is described.
Brain processing, slicing and immunohistochemistry protocol
By onThis protocol is a step by step procedure from collecting brain samples to immunohistochemical staining and mounting brain slices.
Transcriptional analysis of peripheral memory T cells reveals Parkinson’s disease-specific gene signatures–Cell Surface FCS
By onFCS files corresponding to the cell surface experiment described in "Transcriptional analysis of peripheral memory T cells reveals Parkinson’s disease-specific gene signatures"
cDNA clone for bacterial expression of human PPM1H H153D
By onPlasmid for bacterial expression of human PPM1H (H153D); contains TEV protease site for removal of 6His tag.
iPS Cell line: CRICKi012-A (iFCI017), c.G152A mutation in Exon 3 of SNCA
By onMutations in SNCA gene cause rare Parkinson’s disease. iPSC lines from individuals with SNCA G51D mutation were generated successfully, showing normal characteristics. These iPSC lines can aid in studying synucleinopathies for potential therapies.
Optogenetic Manipulation (Mouse)
By onThis protocol describes the steps for in vivo ontogenetic manipulation in mice, including assembly of fiber-ferrules, surgical implantation of fibers, and testing procedure.
Mouse reaching task
By onThe repository includes materials for building and analyzing data from a mouse-reaching task: a bill of materials, 3D models, schematics, an Arduino controller, video recording scripts, and data analysis scripts using DLC.
Border-associated macrophages mediate the neuroinflammatory response in an alpha-synuclein model of Parkinson disease
By onBorder-associated macrophages (BAMs) are crucial in Parkinson's disease pathogenesis by initiating neuroinflammation, highlighting a potential target for therapeutic intervention.
Mouse Ovariectomy
By onThis protocol describes the steps to perform an ovariectomy in a mouse. This procedure is used to induce menopause.
Motor learning selectively strengthens cortical and striatal synapses of motor engram neurons
By onIn this study, Hwang, Roth, et al. find that motor learning recruits a population of engram neurons in the motor cortex that are reactivated during task performance. Motor learning leads to selective remodeling of dendritic spines and strengthening of outputs to the striatum of M1 engram neurons.
The SATB1-MIR22-GBA axis mediates glucocerebroside accumulation inducing a cellular senescence-like phenotype in dopaminergic neurons
By onStudy shows SATB1, MIR22HG, and GBA genes form a pathway in Parkinson's Disease. Dysregulation leads to GluCer accumulation, causing cellular senescence in dopaminergic neurons, potentially explaining neuroinflammation seen in PD and aging.
