Structural and biochemical insights into lipid transport by VPS13 proteins
By onVPS13 proteins are proposed to function at contact sites between organelles as bridges for lipids to move directionally and in bulk between organellar membranes. VPS13s are anchored between membranes via interactions with receptors, including both peripheral and integral membrane proteins. Here we present the crystal structure of VPS13s adaptor binding domain (VAB) complexed with a Pro-X-Pro peptide recognition motif present in one such receptor, the integral membrane protein Mcp1p, and show biochemically that other Pro-X-Pro motifs bind the VAB in the same site. We further demonstrate that Mcp1p and another integral membrane protein that interacts directly with human VPS13A, XK, are scramblases. This finding supports an emerging paradigm of a partnership between bulk lipid transport proteins and scramblases. Scramblases can re-equilibrate lipids between membrane leaflets as lipids are removed from or inserted into the cytosolic leaflet of donor and acceptor organelles, respectively, in the course of protein-mediated transport.
Transfection and protein over-expression
By onThis protocol provides the details for how to transfect and harvest 3X-FLAG-SHIP164Δ901-1099
APEX-Galectin proteomics with and without lysosomal damage
By onProteomics data associated with DOI: 10.7554/eLife.72328
Genome-wide determinants of mortality and clinical progression in Parkinson’s disease – Summary statistics
By onSummary statistics from "Genome-wide determinants of mortality and clinical progression in Parkinson’s disease."
TBK1 knockdown and rescue in Hela-M cells
By onThis protocol transiently depletes TBK1 from HeLa cells and re-introduces a tagged TBK1 along with other relevant components of mitophagy.
Expression and purification of PI3KC3-C1 complex
By onProtocol describing expression and purification of PI3KC3-C1 complex.
Live-cell imaging; mitochondrial reactive oxygen species
By onThis protocol presents instructions for assessing mitochondrial souced reactive oxygen species using MitoTracker® Red CM-H2XRos dye.
Simulations predict differing phase responses to excitation vs. inhibition intheta-resonant pyramidal neurons
By onThis research highlights how cortical neurons respond differently to oscillatory inputs, impacting neuronal responses to network state shifts.
Fast Scan Cyclic Voltammetry (FSCV) in Mouse Brain Slices
By onThis protocol describes the method of fast scan cyclic voltammetry (FSCV) for the application of measuring dopamine transients in mouse brain slices. Within the protocol are sections describing the manufacture of microelectrodes, preparation of acute brain slices, fiber calibration, and measuring stimulated dopamine release.
Intracellular neuromelanin quantification
By onProtocol for intracellular neuromelanin quantification.
Primary data associated with the manuscript “Genome-wide screen reveals Rab12 GTPase as 2 a critical activator of Parkinson’s disease-linked LRRK2 kinase” (3/3)
By onPrimary data associated with the manuscript, "Genome-wide screen reveals Rab12 GTPase as 2 a critical activator of Parkinson’s disease-linked LRRK2 kinase."
Synapsin E-domain is essential for α-synuclein function
By onSynucleins and synapsins are thought to cooperate to regulate synaptic vesicle recycling, but the mechanism is not known. Here we identify the synapsin E-domain as an essential binding partner of α-syn, enabling the function of α-syn at the synapse.
LRRK2 regulates the activation of the unfolded protein response and antigen presentation in macrophages during inflammation
By onAbsence of PINK1 leads to MitAP over-activation engaging autoimmune mechanisms. This pathway is induced by TLR4, cGAS-STING, and UPR activation in response to inflammatory signals. LRRK2 and STING regulate transition from innate to adaptive immunity
Crystal structure ATG9 HDIR in complex with the ATG13:ATG101 HORMA dimer
By onCrystal structure ATG9 HDIR in complex with the ATG13:ATG101 HORMA dimer (Method: X-RAY DIFFRACTION Resolution: 2.41 Å R-Value Free: 0.251 R-Value Work: 0.204 R-Value Observed: 0.205)
