Immunostaining of iPSC-derived neurons
By onThe authors fix, permeabilize, and stain human iPSC-derived neurons for the purpose of observing and quantifying somal and axonal abundance of proteins of interest.
Astrocytic LRRK2 Controls Synaptic Connectivity through ERM Phosphorylation
By onThis study shows that the Lrrk2 gene affects astrocyte morphology by regulating ERM protein phosphorylation, which when reduced, restores synaptic function in PD, suggesting astrocytes as potential therapeutic targets.
Primary neuron culture for live imaging of axonal cargoes
By onThis protocol describes the preparation and culture of mouse primary cortical neurons for live-imaging experiments. Neurons were transfected 16-24 hours before imaging using Lipofectamine 2000.
Neuromelanin staining (Fontana-Masson staining)+ TH-DAB staining on midbrain organoids
By onNeuromelanin staining (Fontana-Masson staining)+ TH-DAB staining on midbrain organoids
Expression and purification protocol of WIPI2d
By onThis protocol details the expression and purification protocol of WIPI2d.
pCAG-MBP-Foldon-ATG9 (828-839)
By onPlasmid: To make ATG9 C-terminal tail trimer for expression in mammalian cells.
H9 ES AAVS1-NGN2::TMEM192-3xHA YIPF4-/-
By onES cells modified to lack YIPF4 Golgi-phagy receptors were used to create iNeurons expressing NEUROG2. CRISPR/Cas9 was employed to introduce the construct in the AAVS1 locus of H9 ES cells.
Global lipidomics of WT and GRN-/- cells & mouse models, including plus and minus GRN addback.
By onThis dataset contains a variety of global lipidomic measurements
The mass spectrometry proteomics data associated of TauRD-Y interactome from TauRD-Y and TauRD-Y* cells.
By onThe mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium with the dataset identifier PXD023400. Associated with the following preprint:
Microscopy-based evaluation of mtKeima flux in hESC-derived Ctrl and FBXO7-/- iNeurons
By onProtocol for the microscopy-based evaluation of mtKeima flux in hESC-derived Ctrl and FBXO7-/- iNeurons PARK15/FBXO7 is dispensable for PINK1/Parkin mitophagy in iNeurons and HeLa cell systems: https://www.embopress.org/doi/full/10.15252/embr.202256399
pCAG-MBP-ATG9 (692-839)
By onPlasmid: To make monomeric ATG9 C-terminal tail for expression in mammalian cells.
Local genetic correlations exist among neurodegenerative and neuropsychiatric diseases
By onGenetic correlation between neurodegenerative and neuropsychiatric diseases was explored using local rg analysis. Unique relationships were found, suggesting shared genetic mechanisms and potential therapeutic targets in complex diseases.
Who is at Risk of Parkinson Disease? Refining the Preclinical Phase of GBA1 and LRRK2 Variant Carriers: a Clinical, Biochemical, and Imaging Approach
By onPurpose of Review Genetic variants in GBA1 and LRRK2 genes are the commonest genetic risk factor for Parkinson disease (PD); however, the preclinical profile of GBA1 and LRRK2 variant carriers who will develop PD is unclear. This review aims to highlight the more sensitive markers that can stratify PD risk in non-manifesting GBA1 and LRRK2 variant carriers. Recent Findings Several case–control and a few longitudinal studies evaluated clinical, biochemical, and neuroimaging markers within cohorts of non-manifesting carriers of GBA1 and LRRK2 variants. Summary Despite similar levels of penetrance of PD in GBA1 and LRRK2 variant carriers (10–30%), these individuals have distinct preclinical profiles. GBA1 variant carriers at higher risk of PD can present with prodromal symptoms suggestive of PD (hyposmia), display increased α-synuclein levels in peripheral blood mononuclear cells, and show dopamine transporter abnormalities. LRRK2 variant carriers at higher risk of PD might show subtle motor abnormalities, but no prodromal symptoms, higher exposure to some environmental factors (non-steroid anti-inflammatory drugs), and peripheral inflammatory profile. This information will help clinicians tailor appropriate screening tests and counseling and facilitate researchers in the development of predictive markers, disease-modifying treatments, and selection of healthy individuals who might benefit from preventive interventions.
Primary neuronal cultures
By onThis protocol is associated with the following preprint, published on February 19th 2022: The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds Itika Saha, Patricia Yuste-Checa, Miguel Da Silva Padilha, Qiang Guo, Roman Körner, Hauke Holthusen, Victoria A. Trinkaus, Irina Dudanova, Rubén Fernández-Busnadiego, Wolfgang Baumeister, David W. Sanders, Saurabh Gautam, Marc I. Diamond, F. Ulrich Hartl, Mark S. Hipp bioRxiv 2022.02.18.481043; doi: https://doi.org/10.1101/2022.02.18.481043
pLenti HsATP10B_D433N-T2A-His-flag-TMEM30A
By onPlasmid: Transfer plasmid for lentiviral vector production expressing Hs ATP10B D433N mutant and His/flag tagged Hs TMEM30A.
Stereology-mediated cell counting using StereoInvestigator
By onProtocol for stereologic count with StereoInvestigator.
Genotyping by next generation sequencing
By onThis collection describes a standard genotyping procedure using next generation sequencing (NGS) in the Hockemeyer lab.
