Transferrin uptake assay to measure Clathrin-mediated endocytosis in HIPCS derived neurons
By onThis protocol is used to measure Transferrin uptake as a way to investigate Clathrin-mediated endocytosis in HIPCS derived neurons, or other cell types.
Structure of human ULK1 complex core (2:2:2 stoichiometry) of the ATG13(450-517) mutant
By onStructure of human ULK1 complex core (2:2:2 stoichiometry) of the ATG13(450-517) mutant
pU6-pegRNA-HEK3-CTTins-px330-scaffold
By onPlasmid: This resource can be used to introduce HEK3 CTT insertion using prime editing. It serves as a good positive control for prime editing.
RHReynolds/neurodegen-psych-local-corr: Code to determine local and global genetic correlations between several neurodegenerative and neuropsychiatric disorders with LAVA and LDSC, respectively.
By onCode for paper "Local genetic correlations exist among neurodegenerative and neuropsychiatric diseases" that explores local genetic correlations among neurodegenerative and neuropsychiatric diseases.
pAC150-Keima-RAMP4
By onThe text describes the use of a PiggyBac vector to express the Keima-RAMP4 autophagy flux reporter, a tool for studying autophagy processes in cells.
Gene expression analysis by qPCR
By onProtocol for gene expression analysis by quantitative Real-Time PCR (qPCR).
Microscopy-based mitochondrial morphology measurements in iNeurons
By onAssociated with preprint: https://www.biorxiv.org/content/10.1101/2022.11.02.514817v1
In Vivo Carbon Fiber Electrode Thread (CFET) Implantation and Testing Procedures
By onProcedures for implanting and testing CFET devices in rats are described.
Immunostaining of iPSC-derived neurons
By onThe authors fix, permeabilize, and stain human iPSC-derived neurons for the purpose of observing and quantifying somal and axonal abundance of proteins of interest.
Astrocytic LRRK2 Controls Synaptic Connectivity through ERM Phosphorylation
By onThis study shows that the Lrrk2 gene affects astrocyte morphology by regulating ERM protein phosphorylation, which when reduced, restores synaptic function in PD, suggesting astrocytes as potential therapeutic targets.
Primary neuron culture for live imaging of axonal cargoes
By onThis protocol describes the preparation and culture of mouse primary cortical neurons for live-imaging experiments. Neurons were transfected 16-24 hours before imaging using Lipofectamine 2000.
Neuromelanin staining (Fontana-Masson staining)+ TH-DAB staining on midbrain organoids
By onNeuromelanin staining (Fontana-Masson staining)+ TH-DAB staining on midbrain organoids
Expression and purification protocol of WIPI2d
By onThis protocol details the expression and purification protocol of WIPI2d.
pCAG-MBP-Foldon-ATG9 (828-839)
By onPlasmid: To make ATG9 C-terminal tail trimer for expression in mammalian cells.
H9 ES AAVS1-NGN2::TMEM192-3xHA YIPF4-/-
By onES cells modified to lack YIPF4 Golgi-phagy receptors were used to create iNeurons expressing NEUROG2. CRISPR/Cas9 was employed to introduce the construct in the AAVS1 locus of H9 ES cells.
Global lipidomics of WT and GRN-/- cells & mouse models, including plus and minus GRN addback.
By onThis dataset contains a variety of global lipidomic measurements
The mass spectrometry proteomics data associated of TauRD-Y interactome from TauRD-Y and TauRD-Y* cells.
By onThe mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium with the dataset identifier PXD023400. Associated with the following preprint:
Microscopy-based evaluation of mtKeima flux in hESC-derived Ctrl and FBXO7-/- iNeurons
By onProtocol for the microscopy-based evaluation of mtKeima flux in hESC-derived Ctrl and FBXO7-/- iNeurons PARK15/FBXO7 is dispensable for PINK1/Parkin mitophagy in iNeurons and HeLa cell systems: https://www.embopress.org/doi/full/10.15252/embr.202256399
