GFP-TBK1: expression and purification
By onThis protocol describes how to express and purify human TBK1 tagged N-terminally with eGFP.
The Hsc70 disaggregation machinery removes monomer units directly from α-synuclein fibril ends
By onUsing microfluidic diffusional sizing, the authors show that the molecular chaperone family Hsp70 (specifically Hsc70, DnaJB, and Apg2) can completely dissolve alpha-synuclein aggregation and revert it back to its monomeric state.
Post mortem human substantial nigra TH staining
By onThis protocol is standardized for postmortem (frozen) SN tissue from pathologically diagnosed PD patients and control individuals for Immunofluorescence staining.
A549 PPM1H-BromoTag (CVCL_C8YV)
By onCell Line: PPM1H-BromoTag CRISPR/CAS9 A549 knock-in cell line generated from parental A549 cells (ATCC A549 CCl-185) by introducing a BromoTag linked to the C-terminus of PPM1H using CRISPR/Cas9 gene-editing technology.
Phenotypic effect of GBA1 variants in individuals with and without Parkinson disease: the RAPSODI study
By onThe authors’ results support previous evidence that GBA1-positive PD has a specific phenotype with more severe non-motor symptoms. The authors did not reproduce previous findings of more frequent prodromal PD signs in non-affected GBA1 carriers.
ImageJ FIJI code to semi-automatize the morphological analysis of microglia in histological sections
By onImageJ FIJI code to semi-automatize the morphological analysis of microglia in histological sections.
Immunohistochemistry of AAV-treated tissues
By onOutlines procedures to perform immunohistochemistry on tissue that had been treated with AAV.
Passaging of hPSCs grown on MEFs
By onThis protocol describes the standard procedure of using collagenase to passage human pluripotent stem cells (hPSCs) on inactivated mouse embryonic fibroblasts (MEFs).
Unilateral intranigral AAV alpha synuclein mouse model
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Intracellular Cytokine (ICS) Staining Protocol
By onThis protocol details about intracellular cytokine (ICS) staining.
H9 ES AAVS1-NGN2 FAM134C/A/B-/-; PiggyBac-Keima-REEP5
By onES cells were modified to create iNeurons lacking FAM134C, A, B receptors and expressing Keima-REEP5 ER-phagy flux reporter. CRISPR/Cas9 was used to introduce NEUROG2 construct in AAVS1 locus and knockout RETREG1, 2, 3 genes.
Fluorescent Immunolabelling for endogenous ChAT in mice striatum
By onThis immunohistochemistry protocol has been designed and adjusted specifically for ChAT immunolabelling in the mice striatum.
Immunofluorescent labeling of cultured cells
By onThis protocol describes the process of Paraformaldehyde fixation and immunofluorescent-labeling of cultured cells.
A feed-forward pathway drives LRRK2 kinase membrane recruitment and activation
By onLRRK2 interacts with Rab8A and Rab10 at specific binding sites, enhancing LRRK2 kinase activity on membranes. This feed-forward pathway regulates LRRK2 activation and substrate phosphorylation.
cDNA clones for expression of GolgiTag (TMEM115) in mammalian cells
By onPlasmids for expression of TMEM115 (GolgiTag) in human and mouse cells.
Evaluation of mtKeima foci in induced neurons (iNeurons)
By onAssociated with preprint: https://www.biorxiv.org/content/10.1101/2022.11.02.514817v1
Piggybac-mediated stable expression of NGN2 in iPSCs for differentiation into excitatory glutamatergic neurons V.2
By onThe authors adapted a previously described method for employing Piggybac transfection to stably express doxycycline-inducible NGN2 in human iPSCs.
