AT8 Tau Pathology Image Analysis
By onThis protocol describes how to analyze AT8 tau pathology in human brain tissue (FFPE sections with IHC).
Coordinating a new approach to basic research into Parkinson’s disease
By onThis article introduces the Aligning Science Across Parkinson's (ASAP) initiative by taking a deep dive into the planning of the initiative, scientific themes, objectives, and outlook.
Quick guide to use paceTOMO for cryo-ET data collection from Titan Krios
By onThis quick guide provides key minimal steps for preparing the Titan/SerialEM for the tomogram data collection on lamella or in vitro specimens with a K3 camera. paceTOMO routine is also included for a typical tomogram data collection session. Please note that this is not an exhaustive guide, but summarises the order of key steps.
Sectioning of Mouse Brain by Microtome
By onThis protocol describes how to use the microtome to prepare and slice mouse brain sections for Immunohistochemistry.
Quantifying Acetylcholinesterase activity in striatal brain tissue with DTNB assay
By onThis protocol describes the steps to measure acetylcholinesterase (AChE) activity in mouse striatal brain tissue, including how to store tissue samples, extract AChE and obtain Michaelis-Menten like plots to measure the rate of AChE activity.
Generation of stable cell lines via retroviral or lentiviral transduction
By onThis protocol details how to generate stable cell lines using a retrovirus system
Global proteomic analysis of WT and GRN-/- HeLa cells
By onGlobal proteomic analysis of WT and GRN-/- HeLa cells https://www.nature.com/articles/s41467-022-33500-9
Neural differentiation on EM grids – iNeurons sample preparation for cryo-ET and CLEM
By onProtocol for direct differentiation of AAVS1-TRE3G-NGN2 iPSCs and hESCs to iNeurons on EM grids: coat grids, seed iNeurons, transduce with fluorescent proteins, then plunge freeze for cryo-ET and cryo-CLEM.
Expression and purification protocol of GST-mCh-FYVE
By onThis protocol details the expression and purification of GST-mCh-FYVE.
Structural basis for ATG9A recruitment to the ULK1 complex in mitophagy initiation
By onHere, the authors examine the structural interaction between ATG9A and components of the ULK1 complex to better understand the process of the PINK1- and Parkin- dependent mitophagy pathway implicated in Parkinson's disease.
Cryo-EM sample preparation
By onThis protocol details how to prepare cryo-EM samples of 3xFLAG-SHIP164Δ901-1099
Midbrain viral injections for striatal fiber photometry in mice
By onThis protocol describes how to: - Inject AAV viruses into the midbrain (SNc: substantia nigra pars compacta, or VTA: ventral tegmental area) of mice - Train mice for head-fixed running on a cylindrical treadmill, and to receive rewards and air puffs while head-fixed - Implant head plates on mice for head-fixation during behavior (optional) While this protocol is focused on injections of AAV viruses into the midbrain, it can be easily modified to inject into other brain regions or other viruses.
Mitochondrial complex activity assays
By onMitochondria complex activity assays measure the activity levels of the different complexes of the mitochondrial electron transport chain (ETC).
Reconstitution of cargo-induced LC3 lipidation in mammalian selective autophagy
By onCode for analysis of multicolor fluorescence images to quantify fluorescence intensity at the perimeter of giant unilamellar vesicles (GUVs).
Immunoblotting of macrophages and microglia
By onThis protocol describes the preparation from cell lysate from cultured cells and immunoblotting procedure.
pCAG-ATG13 (1-197)
By onPlasmid: To make ATG101/ATG13 HORMA complex for expression in mammalian cells.
mCherry-YIPF4 Immunoprecipitation V2
By onThe protocol outlines how to immunoprecipitate mCherry-YIPF4 for further analysis.
