Preparation of an enriched synaptic vesicle fraction
By Emma Sherrell onProcedure for preparing enriched synaptic vesicle fractions used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function."
In vitro dephosphorylation
By Emma Sherrell onProcedure for in vitro dephosphorylation used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function."
In vitro phosphorylation
By Emma Sherrell onProcedure for in vitro phosphorylation used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function."
Proteomics: On-bead/in-solution digestion and phosphopeptide enrichment
By Emma Sherrell onProcedure for On-bead/in-solution digestion and phosphopeptide enrichment used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function."
Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS)
By Emma Sherrell onProcedure for Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS) used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function."
Proteome analysis
By Emma Sherrell onProcedure for proteome analysis used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function."
Multi-electrode array (MEA)
By Emma Sherrell onProcedure for multi-electrode array used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function."
ColabFold protein structure prediction and membrane interaction modeling
By Emma Sherrell onProcedure for ColabFold protein structure prediction and membrane interaction modeling used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function."
Multiple sequence alignment
By Emma Sherrell onProcedure for multiple sequence alignment used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function."
pLP201_pCCL_EFSp_GreenLantern_WPRE
By Emma Sherrell onpLP201_pCCL_EFSp_GreenLantern_WPRE fluorescent reporter plasmid.
pLP202_pCCL_EFSp_mScarlet_WPRE
By Emma Sherrell onpLP202_pCCL_EFSp_mScarlet_WPRE fluorescent reporter plasmid.
Quantification of p62-positive inclusions
By Emma Sherrell onQuantification of p62-positive inclusions in the rodent brain.
Magnetic resonance imaging (MRI) of mouse brain
By Emma Sherrell onMagnetic resonance imaging (MRI) of mouse brain.
Passive avoidance (step-down test) for mice
By Emma Sherrell onPassive avoidance (step-down test) for mice.
Formalin fixed Paraffin Embedded (FFPE) Tissue preparation for digital pathology quantification using QuPath
By Emma Sherrell onQuPath is an open-source digital pathology analysis software. Here, it is used to quantify various misfolded proteins across anatomical regions. This protocol is appropriate for both human and mouse tissue, and will outline the methods of tissue preparation, digitisation, segmentation, and importing images into QuPath.