Optogenetic modulation of dopaminergic neurons
By Emma Sherrell onThis protocol describes an optogenetic activation of neuromelanine-laden dopaminergic neurons in the SNc-VTA.
Chronic optrode recordings from the Locus coeruleus (Optrode construction, implantation, and recording)
By Emma Sherrell onThis protocol covers chronic optrode construction, implantation, and recording in the Locus coeruleus (LC).
H9 ES AAVS1-NGN2 FAM134C-/-; PiggyBac-Keima-RAMP4
By savannah onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor FAM134C and expressing the indicated Keima-RAMP4 ER-phagy flux reporter.
H9 ES AAVS1-NGN2 FAM134C-/-
By savannah onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor FAM134C.
H9 ES AAVS1-NGN2 FAM134B-/-; PiggyBac-Keima-REEP5
By savannah onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor FAM134B and expressing the indicated Keima-REEP5 ER-phagy flux reporter.
H9 ES AAVS1-NGN2 FAM134B-/-; PiggyBac-Keima-RAMP4
By savannah onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor FAM134B and expressing the indicated Keima-RAMP4 ER-phagy flux reporter.
H9 ES AAVS1-NGN2 FAM134B-/-
By savannah onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor FAM134B.
H9 ES AAVS1-NGN2 FAM134A-/-; PiggyBac-Keima-REEP5
By savannah onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor FAM134A and expressing the indicated Keima-REEP5 ER-phagy flux reporter.
H9 ES AAVS1-NGN2 FAM134A-/-; PiggyBac-Keima-RAMP4
By savannah onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor FAM134A and expressing the indicated Keima-RAMP4 ER-phagy flux reporter.
H9 ES AAVS1-NGN2 FAM134A-/-
By savannah onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor FAM134A.
H9 ES AAVS1-NGN2 CCPG1-/-; PiggyBac-Keima-REEP5
By savannah onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor CCPG1 and expressing the indicated Keima-REEP5 ER-phagy flux reporter.
H9 ES AAVS1-NGN2 CCPG1-/-; PiggyBac-Keima-RAMP4
By savannah onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor CCPG1 and expressing the indicated Keima-RAMP4 ER-phagy flux reporter.
H9 ES AAVS1-NGN2 CCPG1-/-
By savannah onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor CCPG1.
A549 PPM1H-BromoTag (CVCL_C8YV)
By savannah onCell Line: PPM1H-BromoTag CRISPR/CAS9 A549 knock-in cell line generated from parental A549 cells (ATCC A549 CCl-185) by introducing a BromoTag linked to the C-terminus of PPM1H using CRISPR/Cas9 gene-editing technology.
HEK293 ::TMEM192-HA YIPF4-/-; YIPF3-/-
By savannah onHEK293 ::TMEM192-HA YIPF4-/-; YIPF3-/- PubMed=37757899.
Circular RNAs in the human brain are tailored to neuron identity and neuropsychiatric disease
By savannah onLittle is known about circular RNAs (circRNAs) in specific brain cells and human neuropsychiatric disease. This study shows that circular RNAs in the human brain are tailored to neuron identity and implicate circRNA-regulated synaptic specialization in neuropsychiatric diseases.
Protocol for Data Independent Acquisition – Mass spectrometry analysis – a DIA-based Organelle Proteomics
By savannah onThis protocol details a Data Independent Acquisition (DIA)-based mass spectrometry (MS) data acquisition method for proteomic profiling of the Golgi, including a description of how to construct the nano Liquid chromatography and DIA MS methods and a Data Dependent Acquisition (DDA) strategy to generate deep spectral libraries for searching the DIA data.
GABAergic regulation of striatal spiny projection neurons depends upon their activity state
By Devin Della Maggiora onPublished: The results demonstrate that GABAARs can work in concert with iGluRs to excite adult SPNs when they are in the resting down-state, suggesting that their inhibitory role is limited to brief periods near spike threshold. This state-dependence calls for a reformulation of the role of intrastriatal GABAergic circuits. View original preprint.
