Protocol outlines precise sample prep for mass spectrometry-based proteomics, emphasizing robust lysis, S-trap column protein capture, and optimized digestion for high-quality data on proteomic changes and post-translational modifications.
Proteomic analysis of iNeurons and cells lacking ER-phagy receptors FAM134C, FAM134A, FAM134B, TEX264, and CCPG1 showed distinct protein profiles, highlighting their roles in ER-phagy pathways.
