Neural differentiation on EM grids – iNeurons sample preparation for cryo-ET and CLEM
Output Details
Description
This is a protocol for differentiating AAVS1-TRE3G-NGN2 iPSCs and hESCs to iNeurons directly on EM grids for cryo-ET and cryo-CLEM. The protocol comprises the following steps:
(1) EM grids coating with Matrigel or Poly-Ornithine and Laminin;
(2) differentiation and seeding of iNeurons on EM grids;
(3) lentiviral transduction for transient expression of fluorescently-labelled proteins;
(4) plunge freezing.
Identifier (DOI)
10.17504/protocols.io.5jyl8jz36g2w/v2
