HEK293T FBXO7-/-
By Julia Julia Leonard onCell Line: HEK293 cells in which FBXO7 has been deleted using CRISPR.
H9 ES AAVS1-NGN2 TEX264-/-; PiggyBac-Keima-REEP5
By Julia Julia Leonard onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor TEX264 and expressing the indicated Keima-REEP5 ER-phagy flux reporter.
H9 ES AAVS1-NGN2 TEX264-/-; PiggyBac-Keima-RAMP4
By Julia Julia Leonard onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor TEX264 and expressing the indicated Keima-RAMP4 ER-phagy flux reporter.
H9 ES AAVS1-NGN2 TEX264-/-
By Julia Julia Leonard onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor TEX264.
H9 ES AAVS1-NGN2 FAM134C/A/B-/-;TEX264-/-; PiggyBac-Keima-REEP5
By Julia Julia Leonard onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor FAM134C, A, B, TEX264, and expressing the indicated Keima-REEP5 ER-phagy flux reporter.
H9 ES AAVS1-NGN2 FAM134C/A/B-/-;TEX264-/-; PiggyBac-Keima-RAMP4
By Julia Julia Leonard onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor FAM134C, A, B, TEX264, and expressing the indicated Keima-RAMP4 ER-phagy flux reporter.
H9 ES AAVS1-NGN2 FAM134C/A/B-/-;TEX264-/-; CCPG1-/-; PiggyBac-Keima-REEP5
By Julia Julia Leonard onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor FAM134C, A, B, TEX264, CCPG1, and expressing the indicated Keima-REEP5 ER-phagy flux reporter.
H9 ES AAVS1-NGN2 FAM134C/A/B-/-;TEX264-/-; CCPG1-/-; PiggyBac-Keima-RAMP4
By Julia Julia Leonard onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor FAM134C, A, B, TEX264, CCPG1, and expressing the indicated Keima-RAMP4 ER-phagy flux reporter.
H9 ES AAVS1-NGN2 FAM134C/A/B-/-;TEX264-/-; CCPG1-/-
By Julia Julia Leonard onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor FAM134C, A, B, TEX264, and CCPG1.
H9 ES AAVS1-NGN2 FAM134C/A/B-/-;TEX264-/-
By Julia Julia Leonard onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor FAM134C, A, B, and TEX264.
H9 ES AAVS1-NGN2 FAM134C/A/B-/-; PiggyBac-Keima-REEP5
By Julia Julia Leonard onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor FAM134C, A, B, and expressing the indicated Keima-REEP5 ER-phagy flux reporter.
H9 ES AAVS1-NGN2 FAM134C/A/B-/-; PiggyBac-Keima-RAMP4
By Julia Julia Leonard onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor FAM134C, A, B, and expressing the indicated Keima-RAMP4 ER-phagy flux reporter.
H9 ES AAVS1-NGN2 FAM134C/A/B-/-
By Julia Julia Leonard onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor FAM134C, A, and B.
H9 ES AAVS1-NGN2 FAM134C/A-/-; PiggyBac-Keima-REEP5
By Julia Julia Leonard onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor FAM134C, A, and expressing the indicated Keima-REEP5 ER-phagy flux reporter.
H9 ES AAVS1-NGN2 FAM134C/A-/-; PiggyBac-Keima-RAMP4
By Julia Julia Leonard onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor FAM134C, A, and expressing the indicated Keima-RAMP4 ER-phagy flux reporter.
H9 ES AAVS1-NGN2 FAM134C/A-/-
By Julia Julia Leonard onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor FAM134C & A.
H9 ES AAVS1-NGN2 FAM134C-/-; PiggyBac-Keima-REEP5
By Julia Julia Leonard onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor FAM134C and expressing the indicated Keima-REEP5 ER-phagy flux reporter.
SOP for TriZOL RNA and protein extraction
By Emma Sherrell onSOP for TriZOL RNA and protein extraction.
