Protein network analysis links the NSL complex to Parkinson’s disease via mitochondrial and nuclear biology
By onA bioinformatics approach was taken to investigate the proteome of the NSL complex, to unpick its relevance to PD progression. The authors’ data points to NSL complex members OGT and WDR5 as key drivers of this increased PD association.
Expression and purification protocol of ULK1 Complex wt or K46I mutant
By onThis Protocol describes the expression and purification of ULK1 Complex wt or K46I mutant.
HEK293 TMEM192-HA ::mNEON-YIPF4
By onHEK293 TMEM192-HA ::mNEON-YIPF4 HEK293::TMEM192-3xHA::mNEON-YIPF4 PubMed=37757899; Characteristics: Using CRISPR/Cas9 TMEM192 was C-terminally tagged on both alleles with a 3xHA epitope (from parent cell line). Characteristics: Using CRISPR/Cas9 YIPF4 was N-terminally tagged on both alleles with mNeonGreen (PubMed=37757899). Transfected with: UniProtKB; A0A1S4NYF2; mNeonGreen (derivative of Branchiostoma lanceolatum blFP-Y3). Transformant: NCBI_TaxID; 28285; Adenovirus 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. NCBI_TaxID=9606; ! Homo sapiens (Human) RRID:CVCL_C0I5 ! HEK293::TMEM192-3xHA Female Fetus Transformed cell line
Purification of recombinant TauRD from E. coli
By onThe protocol outlines procedures for studying Tau protein aggregation in vitro using ThT fluorescence measurements.
A Single-Cell Atlas of Cell Types, States, and Other Transcriptional Patterns from Nine Regions of the Adult Mouse Brain
By onWe report 690K single-cell transcriptomes from nine different brain regions from adult mice (Frontal and Posterior Cortex, Hippocampus, Thalamus, Cerebellum, Striatum, Globus Pallidus externus/Nucleus Basalis, Entopeduncular / Subthalamic Nuclei, & Substantia Nigra / Ventral Tegmental Area).
fcs files to “The extracellular chaperone Clusterin enhances Tau aggregate seeding in a cellular model”
By onDataset associated with DOI: 10.17617/3.51VXU0
Gcase co-immunoprecipitation
By onThe authors developed this protocol to identify protein-protein interactions between the enzyme glucocerebrosidase (GCase) and other proteins in human iPSC-derived Neural Precursor Cells.
pCAG-mcherry- WIPI2dK128E- cs-TEV -STREP
By onPlasmid for mammalian expression of human WIPI2d K128E with N-terminal mCherry and C-terminal Strep.
Raw tomogram of endogenous a-synuclein inclusion in primary mouse neurons seeded with PFFs
By onRaw data associated with DOI: 10.1038/s41467-021-22108-0
mCh-Miro1(∆TM)-SspB
By onmCh-Miro1(∆TM)-SspB plasmid has a deletion of transmembrane domain and is used for improved light inducible dimer assay
In vivo reduction of age-dependent neuromelanin accumulation mitigates features of Parkinson’s disease
By onUsing a newly developed rodent model, the authors assessed whether the intracellular buildup of neuromelanin that occurs with age can be slowed down in vivo to prevent or attenuate Parkinson’s disease.
pBPK1520-SNCA-A30P-ng
By onIt can be used to introduce SNCA-A30P mutation using prime editing, PE3 approach.
In situ architecture of neuronal α-Synuclein inclusions
By onAlpha-synuclein aggregation has been associated with Parkinson’s disease. Using cutting-edge imaging tools, the authors show neuronal alpha-synuclein inclusions within their native states.
katiekellyucl/W-PPI-NA-NSL-complex: v1.0.0. W-PPI-NA/NSL_complex
By onSoftware for in Silico analysis linking the NSL complex to Parkinson’s disease and the mitochondria (protein-protein interaction data to functional enrichment analysis)
The extracellular chaperone Clusterin enhances Tau aggregate seeding in a cellular model
By onTau neuronal aggregation is a driver of some neurodegenerative disorders. The authors show that a protein,Clusterin, delays Tau aggregation and suppresses seeding activity.
