Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function
By onRaw data files used for the manuscript "Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function" https://www.researchsquare.com/article/rs-1521848/v1
HeLa ::TMEM192-HA YIPF3-/-
By onHeLa ::TMEM192-HA YIPF3-/- PubMed=37757899; Population: African American. Characteristics: Using CRISPR/Cas9 TMEM192 was C-terminally tagged on both alleles with a 3xHA epitope (from parent cell line). Knockout cell: Method=CRISPR/Cas9; HGNC; 21023; YIPF3. Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18). Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002. NCIt; C27677; Human papillomavirus-related cervical adenocarcinoma NCBI_TaxID=9606; ! Homo sapiens (Human) RRID:CVCL_D1KR ! HeLa::TMEM192-3xHA Female 30Y6M Cancer cell line
Histology and Retention of Implanted Carbon Fiber Thread Electrodes in Fixed Tissue
By onMethods to cut and retain implanted carbon fiber electrodes in brain tissue.
EPSCs source data across different simulation intensities of thalamus PT, IT neurons, and SC PT neurons
By onSource data compares EPSCs in thalamus PT, IT neurons, and SC PT neurons between control and 6_OHD mice across varying simulation intensities.
Transcardiac Perfusion of Mouse for Brain Tissue
By onTranscardiac perfusion is a method used for clearing blood and preserving the mouse brain for immunostaining.
PARK15/FBXO7 is dispensable for PINK1/Parkin mitophagy in iNeurons and HeLa cell systems
By onPINK1 and Parkin promote damaged mitochondria removal through Ub phosphorylation, Parkin activation. FBXO7 mutation in PD may not affect mitophagy in HeLa and neuron cells, suggesting it may not play a significant role in Parkin-dependent mitophagy.
Primary data associated with the manuscript “Golgi-IP, a novel tool for multimodal analysis of Golgi molecular content” (doi.org/10.1101/2022.11.22.517583)
By onRaw data files used for the manuscript "Golgi-IP, a novel tool for multimodal analysis of Golgi molecular content" (doi.org/10.1101/2022.11.22.517583).
Expression and purification for cryo-EM samples of FIP200NTD:ATG13(363-517)-ULK1(MIT) complexes
By onProtocol describing expression and purification protocols for cryo-EM samples of FIP200NTD:ATG13(363-517)-ULK1(MIT) complexes.
LRRK2 microtubule sedimentation binding assay
By onAssay to determine LRRK2 protein binding to microtubules
Bulk RNA sequencing analysis
By onThis protocol describes the steps for the bioinformatical analysis of bulk RNA sequencing with a focus on evolutionary young L1s.
Protein aggregation and calcium dysregulation are hallmarks of familial Parkinson’s disease in midbrain dopaminergic neurons
By onMutations in SNCA gene cause PD by forming α-synuclein aggregates. Using hiPSCs, we traced pathophysiological events, revealing early oligomeric aggregate formation, calcium signaling impairments, and multiple cellular stresses leading to cell death.
In vitro phosphatase assay
By onThis protocol describes the procedure to perform in vitro phosphatase assay.
Collection of protocols of Team Deleidi used in the publication: “”LRRK2 kinase activity regulates GCase level and enzymatic activity differently depending on cell type in Parkinson’s disease””
By onCollection of protocols of Team Deleidi used in the publication: ""LRRK2 kinase activity regulates GCase level and enzymatic activity differently depending on cell type in Parkinson’s disease""
GUV assay
By onThis protocol describes the preparation of GUVs for the reconstitution of LC3 lipidation reaction through the steps of PI3P production by PI3KC3-C1, WIPI2 recruitment and LC3 lipidation with PE. Screen reader support enabled.
pFastBac_Dual_GST-TEV-mCherry-TBK1
By onPlasmid: Plasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631. Used for the expression and purification of mCherry labelled TBK1 (SMC Internal No.: 1675).
LRRK2RCKW Widefield fluorescence microtubule binding assay
By onThis assay uses TMR labeled LRRK2 or LRRK1 RCKW to measure binding to microtubules in vitro
Metabolomic and lipidomic data reported in doi.org/10.1073/pnas.2219953120 (Golgi-IP, a tool for multimodal analysis of Golgi molecular content)
By onMetabolomic and lipidomic data associated with doi.org/10.1073/pnas.2219953120 deposited in MetaboLights repository with identifier number MTBLS6511.
Golgi-IP, a novel tool for multimodal analysis of Golgi molecular content
By onThe authors develop a method for the rapid capture of intact Golgi via Golgi immunoprecipitation. Using high-resolution mass spectrometry, the approach allows the unbiased characterization of the Golgi proteome, metabolome, and lipidome.
