pH-Rodo pathogens to monitor phagocytosis in pMacs
By Julia Julia Leonard onpH-Rodo pathogens to monitor phagocytosis in pMacs.
DQ-BSA and BMV109 microscopy
By Julia Julia Leonard onDQ-BSA and BMV109 microscopy for peritoneal macrophages.
Nigrostriatal Tau pathology in Parkinsonism and Parkinson’s disease
By Emma Sherrell onDataset corresponding to the preprint, "Is Tau the Initial Pathology in Dopaminergic Nigrostriatal Degeneration? Studies in Parkinsonism and Parkinson’s Disease."
Structural analysis of 20S CPs and assembly intermediates by electron cryo-microscopy
By Emma Sherrell onThis protocol details methods for structural determination by transmission electron cryo-microscopy of 20S CPs and assembly intermediates.
Flow cytometry for ex vivo stimulated pMacs
By Julia Julia Leonard onFlow cytometry for ex vivo stimulated pMacs.
Cloning, protein expression, and purification of 20S CPs and assembly intermediates
By Emma Sherrell onThis protocol details methods for cloning, expression, and purification of 20S CPs, and assembly intermediates for biochemical and structural analysis.
Open-source platform for kinematic analysis of mouse forelimb movement
By Emma Sherrell onAn open-source behavioral platform and software solution for studying fine motor skills in mice performing reach-to-grasp tasks. The behavioral platform uses readily available and 3D-printed components and was designed to be affordable and universally reproducible.
3D bioprinting of human neural tissues with functional connectivity
By Emma Sherrell onProbing how human neural networks operate is hindered by the lack of reliable human neural tissues amenable to the dynamic functional assessment of neural circuits. Team Scherzer developed a 3D bioprinting platform to assemble tissues with defined human neural cell types in a desired dimension using a commercial bioprinter.
Visualizing chaperone-mediated multistep assembly of the human 20S proteasome
By Emma Sherrell onDedicated assembly factors orchestrate the stepwise production of molecular machines, including the 28-subunit proteasome core particle (CP) that mediates protein degradation. This article presents cryo-EM reconstructions of seven recombinant human subcomplexes that visualize all five chaperones and the three active site propeptides across a wide swath of the assembly pathway. View original preprint.
Tau aggregation monitored by thioflavin-T (ThT) fluorescence in a plate reader
By Emma Sherrell onThis protocol details how to efficiently monitor Tau aggregation by thioflavin-T fluorescence in a plate reader.
Solid phase binding assay – Clusterin binding to very low-density lipoprotein receptor (VLDLR)
By Emma Sherrell onThis protocol details how to monitor Clusterin binding to the very low-density lipoprotein receptor (VLDLR) by enzyme-linked immunosorbent assays (ELISA) adapted from Leeb et al., 2014.
Amyloid beta (Aβ) aggregates N-terminal labeling
By Emma Sherrell onThis protocol details how to efficiently label protein aggregates at the N-terminal using Amyloid beta as example.
Harvesting and culturing of peritoneal macrophages and ex vivo stimulation
By Julia Julia Leonard onHarvesting and culturing of peritoneal macrophages and ex vivo stimulation with IFNy.
α-Synuclein aggregation monitored by thioflavin-T (ThT) fluorescence in a plate reader
By Emma Sherrell onThis protocol details how to efficiently monitor α-Synuclein aggregation by thioflavin-T fluorescence in a plate reader.
GFP-Clu-tails purification from Escherichia coli cells
By Emma Sherrell onThis protocol details how to purify the fusion protein GFP-Clu-tails from Escherichia coli.
MBP-Clu-tail purification from Escherichia coli cells
By Emma Sherrell onThis protocol details how to efficiently purify the fusion protein Maltose binding protein (MBP)-Clu-tail (204-238) from Escherichia coli.
Rat organotypic cultures for AAV-mediated vital labeling of the extracellular matrix V.2
By Julia Julia Leonard onIn this protocol, the authors describe the preparation and maintenance of rat organotypic cultures (cortico-striatal) for AAV infection and imaging of the extracellular matrix.
Polyglucosan body density in the aged mouse hippocampus is controlled by a novel modifier locus on chromosome 1
By Emma Sherrell onAging can be associated with the accumulation of polyglucosan bodies (PGBs). While PGBs have a detrimental effect on cognition, the underlying mechanism and clinical relevance of age-related PGB accumulation remain unknown. Here, the authors investigated the genetic basis and functional impact of age-related PGB accumulation in mice.
Transcriptional programs mediating neuronal toxicity and altered glial-neuronal signaling in a Drosophila knock-in tauopathy model
By Emma Sherrell onPublished: Mutations in gene encoding tau cause autosomal dominant forms of frontotemporal dementia. Prior models of frontotemporal dementia replicate features of the disease but do not recreate the genetic context. This article presents a genetic model of tauopathy, which recapitulates the genetic context and phenotypic features of the disease. View original preprint.
