Determination of NM Concentration
By onThis is the protocol for determining neuromelanin concentration and data.
cDNA clones for expression of LysoTag (TMEM192) in mammalian cells
By onPlasmids for expression of TMEM192 (LysoTag) in human and mouse cells.
Immunohistochemistry for Carbon Fiber Thread Electrodes
By onMethods for immunofluorescent staining of brain tissue with indwelling electrodes.
pCAG-OSF-ATG13 (2-197)-K15D
By onPlasmid for expression of human ATG13 HORMA K15D mutant in mammalian cells.
Nuclear Isolation of Post-Mortem Brain Tissue for snRNAseq
By onThis protocol details how to isolate nuclei from frozen brain tissue for single nuclear RNA sequencing using 10x Genomics GEM isolation using the Chromium accessory and Single Cell 3ʹ Reagent Kits.
3D-correlative FIB-milling and Cryo-ET of Autophagic structures in Yeast Cells
By onThis protocol describes how to plunge-freeze yeast on EM grids and how to target autophagic structures by combining cryo confocal fluorescence data to FIB-milling and tomogram acquisition.
Expansion Microscopy
By onExpansion microscopy is a technique to visualize biological structures with higher spatial resolution than traditional microscopy methods.
Immunofluorescent staining for neuronal marker MAP2
By onThis is the protocol for immunofluorescent staining for neuronal marker MAP2.
circRNA dataset
By onNew data set on circRNA in laser-captured neuron samples from 190 human brains of healthy controls, prodromal PD, and clinical PD (Dong et al., Nature Communications, in press). The RNA-seq raw FASTQ data and normalized expression matrix of all circRNAs in this study have been deposited in GEO under accession number GSE218203.
circRNA Custom Code
By onCustom code associated with (Dong et al., Nature Communications, in press) is publicly available at https://github.com/sterding/circRNA.
Unconventional Initiation of PINK1/Parkin Mitophagy by Optineurin
By onCargo sequestration is a fundamental step of selective autophagy in which cells generate a double membrane structure termed an autophagosome on the surface of cargoes. The authors uncover an unconventional path of PINK1/Parkin mitophagy initiation.
Microscopy-based GSH bead protein-protein interaction assay
By onProtocol describing the Microscopy-based GSH bead protein-protein interaction assay
Cellular lipid uptake with flow cytometry readout
By onCellular lipid uptake with flow cytometry readout
Global and APEX proteomics studying Golgi-phagy
By onDataset collection includes experiments on HEK, HeLa, iNeurons, and H9 ES cells under nutrient stress. Various proteomics analyses were conducted with different protein factors like FIP200, ATG7, LIR binding sites, YIPF4, YIPF3, and CALCOCO1.
Cell lysis and immunoblotting for protein and phospho-protein quantification
By onThe authors describe the procedure by which human iPSC-derived neurons or mouse embryonic fibroblasts (MEFs) were lysed and probed for levels of proteins of interest using Western blot.
