Quantification of area and optical density of intracellular neuromelanin with Image J
By Julia Julia Leonard onQuantification of area and optical density of intracellular neuromelanin with Image J.
Protocol for preparing post-mortem tissue for intracellular neuromelanin quantification in H&E-stained sections
By Julia Julia Leonard onProtocol for preparing post-mortem tissue for intracellular neuromelanin quantification in H&E-stained sections.
Quantification of area and optical density of intracellular neuromelanin with TruAI in H&E stained sections
By Julia Julia Leonard onQuantification of area and optical density of intracellular neuromelanin with TruAI in H&E stained sections.
Toward a standard model for autophagosome biogenesis
By Emma Sherrell onTwo papers in this issue resolve a long-standing obstacle to a “standard model” for autophagosome biogenesis in mammals.
The annotation of GBA1 has been concealed by its protein-coding pseudogene GBAP1
By Emma Sherrell onPublished: The authors identify novel transcripts from both GBA1 and GBAP1, including protein-coding transcripts that are translated in vitro and detected in proteomic data, but that lack GCase activity. View original preprint.
Regulation of mitophagy by the NSL complex underlies genetic risk for Parkinson’s disease: Bioinformatic prioritization and hit validation
By Emma Sherrell onThis protocol describes the Bioinformatic Prioritization of PD GWAS candidates for High Content Screening, and Hit Validation by allele-specific expression (ASE) analysis.
Primary data associated with the manuscript, “Genome-wide screen reveals Rab12 GTPase as 2 a critical activator of Parkinson’s disease-linked LRRK2 kinase” (3/3)
By Emma Sherrell onPrimary data associated with the manuscript, "Genome-wide screen reveals Rab12 GTPase as 2 a critical activator of Parkinson’s disease-linked LRRK2 kinase."
Primary data associated with the manuscript, “Genome-wide screen reveals Rab12 GTPase as 2 a critical activator of Parkinson’s disease-linked LRRK2 kinase” (2/3)
By Emma Sherrell onPrimary data associated with the manuscript, "Genome-wide screen reveals Rab12 GTPase as 2 a critical activator of Parkinson’s disease-linked LRRK2 kinase." These include raw immunoblotting data (.tiff files), annotated images (.pdf), and their quantitation and graphs obtained using GraphPad Prism(.pzf files). These also include microscopy images and their analyses in GraphPad Prism (.pzf files).
Primary data associated with the manuscript, “Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55B” (doi: 10.1101/2023.06.07.544051)
By Emma Sherrell onConfocal microscopy images, CellProfiler Excel files of Pearson's coefficients between TMEM55B/RILPL1 or pRab10/RILPL1 in R1441C MEF or VPS35 MEF cells, and Prism files of graphed Pearson's coefficients for each condition, as seen in doi: 10.1101/2023.06.07.544051.
In vivo carbon fiber electrode thread (CFET) implantation and testing procedures
By Emma Sherrell onProcedures for implanting and testing CFET devices in rats are described.
In vitro FSCV testing of carbon fiber electrodes to characterize functional operation in dopamine detection
By Emma Sherrell onMethods to measure the performance characteristics of carbon fiber electrodes for neurochemical recording.
Immunohistochemistry for carbon fiber thread electrodes
By Emma Sherrell onMethods for immunofluorescent staining of brain tissue with indwelling electrodes.
Immunofluorescent staining for neuronal marker MAP2
By Emma Sherrell onThis is the protocol for immunofluorescent staining for neuronal marker MAP2.
Human neuroblastoma cell line SH-SY5Y culturing
By Emma Sherrell onThis is the brief protocol for seeding and culturing human neuroblastoma SH-SY5Y cells.
Histology and retention of implanted carbon fiber thread electrodes in fixed tissue
By Emma Sherrell onMethods to cut and retain implanted carbon fiber electrodes in brain tissue.
HEK293T SNCA-A53T-mRuby3 SNCA-A53T-mClover3
By Emma Sherrell onCell Line: Transfected with two plasmids encoding mRuby3- and mClover3-tagged SNCA p.Ala53Thr expressed under the control of CMV promoters.
HEK293E_Ecto short VLDLR-C-tag
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing the ectodomain of VLDLR fused to a C-terminal C-tag (EPEA) under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu WT
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu TL4
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu TL3
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
