all protocols related to “Spermidine suppresses glial inflammation and parkinsonian abnormalities in ATP13A2 deficiency”
By onAll protocols related to "Spermidine suppresses glial inflammation and parkinsonian abnormalities in ATP13A2 deficiency"
Regulation of the proteasome 20S core particle by the Parkinsonism-associated Proteins FBXO7 and PI31
By onGenetic forms of PD are associated with variants in FBXO7 and PI31. Combining biochemical and structural approaches, we describe the proteasome interaction of these proteins and map disease variants to disruption of proteasome interaction.
Cell-autonomous and non-cell-autonomous drivers of dopamine neuron vulnerability in Parkinson’s disease
By onThis review examines evidence for cell-autonomous mechanisms and non-cell-autonomous processes arising from glial and peripheral immune cells and how these pathways can converge to create chronic cellular stress and trigger cell death mechanisms.
TEsingle Software
By onSoftware to analyze TE expression in single cell/single nuclei RNA-seq datasets.
TEsingle pipeline for analysis of Martirosyan dataset
By onCode to analyze Martirosyan et al. substantia nigra single nuclei RNA-seq dataset with TEsingle.
TEsingle benchmarking pipeline
By onCode to perform benchmarking of simulated single cell/nuclei RNA-seq datasets with TEsingle and other scRNA-seq software.
Simulation data for TEsingle benchmarking
By onThis dataset includes files for benchmarking analysis with TEsingle and other snRNA tools, facilitating reproducibility.
Fluorescence-Gated Flow Cytometry Approach for Measuring Lipid Flippase Activity in Mammalian Cells
By onP4-ATPase lipid flippases create lipid asymmetry in cells. A new strategy using NBD-lipid uptake assays improves sensitivity and analysis of ATP11C function. This method enhances studying regulatory interactions in mammalian cells.
Data and code for Active Escape (“Integrated Representations of Threat and Control in the Lateral Frontal Pole”)
By onData, Code, and Key Resource Table used in the preprint from Stasiak et al., 2025 ("Integrated Representations of Threat and Control in the Lateral Frontal Pole").
Active Escape Protocol (Stasiak et al., 2026)
By onProtocol for Active Escape task by Stasiak et al., referenced in "Integrated Representations of Threat and Control in the Lateral Frontal Pole" (2026) manuscript.
pCHMWS-hATP13A4_A356V-IRES-Puro
By ontransfer plasmid for lentiviral vector production expressing Hs ATP13A4 A356V mutant
pCHMWS-hATP13A4_A356V-Twinstrep-Flag-IRES-Puro
By ontransfer plasmid for lentiviral vector production expressing Hs ATP13A4 A356V mutant with Twinstrep-Flag tag
AAV-gfaABC1D-Lamp1-TurboID-HA
By onUsing the gfaABC1D promoter expressing Lamp1-TurboID fusion protein
pCHMWS-FLUC-IRES-Puro
By ontransfer plasmid for lentiviral vector production expressing firefly luciferase (FLUC)
AAV-gfaABC1D-cytoTurboID-HA
By onUsing the gfaABC1D promoter expressing cytoplasmic TurboID with C-terminal HA tag
Postnatal astrocyte labeling by electroporation
By onHere, we provide a step-by-step guide to perform Postnatal Astrocyte Labeling by Electroporation (PALE), detailing the preparation, electroporation procedure, and post-electroporation analysis.
in vivo TurboID
By onProtocol uses iBioID with Lamp1-TurboID in astrocytes to study endo-/lysosomal protein interactions in mice. AAV delivery allows selective biotinylation for proteome mapping, offering insights into astrocyte signaling in vivo.
Immunocytochemistry – subcellular localization of ATP13A4
By onThis protocol describes an optimized method for visualizing the subcellular localization of over-expressed ATP13A4 in HeLa cells using immunocytochemistry.
IHC staining (DAB) of Iba1 and Gfap
By onThis protocol details the IHC staining (DAB) of Iba1 and Gfap.
