Collaborative Research Network Archive

A sensitive method to analyze the full complement of GSL structures present in mammalian cells, fluids, and tissues.

Protocol for neuronal activation of the enteric nervous system.

Protocol for immunophenotyping peripheral blood immune cells.

Protocol to assess cellular phenotypes by multiparametric flow cytometry panels containing markers to identify cell types and activation states.

Protocol for rat brain processing to perform parallel histological analysis in one hemisphere and high dimensional flow cytometry in the other.

This protocol is a quick guide to how to prepare a solution of LPS on the saline vehicle, how to inject a rat for an intraperitoneal injection, and how to monitor the animal after injection.

This protocol describes preparation of organotypic hemisphere cultures prepared from mouse brain and cultured as rollerdrum cultures on glass coverslips.

Protocol for preparing post-mortem tissue for intracellular NM quantification.

Quantification of area and optical density of intracellular neuromelanin with TruAI.

Designing and properly mounting FFPE sections on large-window CytAssist slides is important for downstream analysis and data quality. This protocol describes the key steps for this preparation.

This is the protocol to scan brain neuromelanin with reduced impact of the chemicals on their original color and intensity. The immunofluorescence staining protocol is designed to identify human A6, A9, and A10 cell clusters and alpha-synuclein pathology.

This protocol details acute extracellular multi-unit recordings and optotagging in mice.

This protocol describes an automatizable pipeline for cell counting based on fluorescent stainings with the help of CellPose. Additionally, it describes the entire generation process of plots that show the intensity of neuromelanin per cell based on bright-field images.