HEK293 TMEM192-HA ::mNEON-YIPF4
By onHEK293 TMEM192-HA ::mNEON-YIPF4 HEK293::TMEM192-3xHA::mNEON-YIPF4 PubMed=37757899; Characteristics: Using CRISPR/Cas9 TMEM192 was C-terminally tagged on both alleles with a 3xHA epitope (from parent cell line). Characteristics: Using CRISPR/Cas9 YIPF4 was N-terminally tagged on both alleles with mNeonGreen (PubMed=37757899). Transfected with: UniProtKB; A0A1S4NYF2; mNeonGreen (derivative of Branchiostoma lanceolatum blFP-Y3). Transformant: NCBI_TaxID; 28285; Adenovirus 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. NCBI_TaxID=9606; ! Homo sapiens (Human) RRID:CVCL_C0I5 ! HEK293::TMEM192-3xHA Female Fetus Transformed cell line
Purification of recombinant TauRD from E. coli
By onThe protocol outlines procedures for studying Tau protein aggregation in vitro using ThT fluorescence measurements.
A Single-Cell Atlas of Cell Types, States, and Other Transcriptional Patterns from Nine Regions of the Adult Mouse Brain
By onWe report 690K single-cell transcriptomes from nine different brain regions from adult mice (Frontal and Posterior Cortex, Hippocampus, Thalamus, Cerebellum, Striatum, Globus Pallidus externus/Nucleus Basalis, Entopeduncular / Subthalamic Nuclei, & Substantia Nigra / Ventral Tegmental Area).
fcs files to “The extracellular chaperone Clusterin enhances Tau aggregate seeding in a cellular model”
By onDataset associated with DOI: 10.17617/3.51VXU0
Gcase co-immunoprecipitation
By onThe authors developed this protocol to identify protein-protein interactions between the enzyme glucocerebrosidase (GCase) and other proteins in human iPSC-derived Neural Precursor Cells.
pCAG-mcherry- WIPI2dK128E- cs-TEV -STREP
By onPlasmid for mammalian expression of human WIPI2d K128E with N-terminal mCherry and C-terminal Strep.
Raw tomogram of endogenous a-synuclein inclusion in primary mouse neurons seeded with PFFs
By onRaw data associated with DOI: 10.1038/s41467-021-22108-0
mCh-Miro1(∆TM)-SspB
By onmCh-Miro1(∆TM)-SspB plasmid has a deletion of transmembrane domain and is used for improved light inducible dimer assay
In vivo reduction of age-dependent neuromelanin accumulation mitigates features of Parkinson’s disease
By onUsing a newly developed rodent model, the authors assessed whether the intracellular buildup of neuromelanin that occurs with age can be slowed down in vivo to prevent or attenuate Parkinson’s disease.
pBPK1520-SNCA-A30P-ng
By onIt can be used to introduce SNCA-A30P mutation using prime editing, PE3 approach.
In situ architecture of neuronal α-Synuclein inclusions
By onAlpha-synuclein aggregation has been associated with Parkinson’s disease. Using cutting-edge imaging tools, the authors show neuronal alpha-synuclein inclusions within their native states.
katiekellyucl/W-PPI-NA-NSL-complex: v1.0.0. W-PPI-NA/NSL_complex
By onSoftware for in Silico analysis linking the NSL complex to Parkinson’s disease and the mitochondria (protein-protein interaction data to functional enrichment analysis)
The extracellular chaperone Clusterin enhances Tau aggregate seeding in a cellular model
By onTau neuronal aggregation is a driver of some neurodegenerative disorders. The authors show that a protein,Clusterin, delays Tau aggregation and suppresses seeding activity.
Introducing GolgiTag to Cells and Immunoprecipitation of Golgi
By onProtocol for generating GolgiTag cells and immunoprecipitation of Golgi for subsequent -omics analyses.
