His-ATG3-R265A
By Emma Sherrell onPlasmid: ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-R265A-stop.
His-ATG3-H266A
By Emma Sherrell onPlasmid: ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-H266A-stop.
His-ATG3-K208D
By Emma Sherrell onPlasmid: ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-K208D-stop.
His-ATG3-K62D/K64D
By Emma Sherrell onPlasmid: ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-K62D/K64D-stop.
Oncogenic BRAF V600E induces glial proliferation through ERK and neuronal death through JNK
By Emma Sherrell onActivating V600E in BRAF is a common driver mutation in cancers of multiple tissue origins. BRAF V600E has also been implicated in neurodegeneration. The present study aims to characterize BRAF V600E on cell death and survival in three major cell types of the CNS: neurons, astrocytes, and microglia.
T cell differentiation from mice spleen tissue
By Emma Sherrell onThis protocol is for T cell differentiation from mice spleen tissue to investigate T cell function (including differentiation and proliferation) in vitro.
Genetic modification of human induced pluripotent stem cells (hiPSCs) by lentiviral transduction protocol
By Emma Sherrell onThis is the protocol for the genetic modification of human induced pluripotent stem cells (hiPSCs) using lentiviral transduction method.
Cytotoxicity Assay Protocol
By Emma Sherrell onThis is the protocol for cytotoxicity assay in cancer cell lines using flow cytometry.
Preparation of Holding ACSF (artificial cerebrospinal fluid) for slice electrophysiology
By Emma Sherrell onHolding ACSF is used for incubating brain slices before cell-attached or whole-cell electrophysiological recordings of neurons.
Preparation of Recording ACSF (artificial cerebrospinal fluid) for slice electrophysiology
By Emma Sherrell onRecording ACSF is used for incubating brain slices during cell-attached or whole-cell electrophysiological recordings of neurons.
v2 RNAscope
By Emma Sherrell onThis is a protocol for performing RNAscope® in situ hybridization analysis on fixed-frozen mouse brain tissue using the RNAscope® Multiplex Fluorescent v2 kit (Advanced Cell Diagnostics; ACD).
Integrative analysis reveals a conserved role for the amyloid precursor protein in proteostasis during aging
By Emma Sherrell onAβ peptides derived from the amyloid precursor protein (APP) have been implicated in the pathogenesis of Alzheimer’s disease. However, the normal function of APP is less clear. Results demonstrate a conserved role for APP in controlling age-dependent proteostasis with plausible relevance to Alzheimer’s disease.
Imaging and analysis of mouse midbrain tissue labeled by RNAscope
By Emma Sherrell onThe authors describe imaging, analysis, and cell counts of coronal sections of mouse substantia nigra and ventral tegmental area labeled with Slc17a6 (VGLUT2), Slc32a1 (VGAT), and Slc18a2 (VMAT2).
Preparation and RNAscope-labeling of fresh mouse midbrain tissue
By Emma Sherrell onThe authors describe preparing and labeling coronal sections of mouse substantia nigra (SNc) and ventral tegmental area (VTA) for probes targeting the genes Slc17a6 (VGLUT2), Slc32a1 (VGAT), and Slc18a2 (VMAT2).
LC/MS analysis of plasma samples from PPMI
By Emma Sherrell onPlasma samples from PPMI were analyzed by liquid chromatography with mass spectrometry (LC/MS) for a variety of metabolites (including piperine) and lipids as interrelated markers of Parkinson's disease and its pathophysiology.
Modeling gene-environment interactions in Parkinson’s disease: Helicobacter pylori infection of Pink1-/- mice induces CD8 T cell-dependent motor and cognitive dysfunction
By Emma Sherrell onParkinson's disease (PD) is a neurodegenerative disorder characterized by loss of motor function. Using a mouse model, the authors demonstrate that infection with Helicobacter pylori leads to the development of motor and cognitive signs.
Purification of total RNA from cells using spin technology
By Emma Sherrell onPurification of total RNA from cells using spin technology.
Large-scale visualization of α-synuclein oligomers in Parkinson’s disease brain tissue
By Emma Sherrell onParkinson’s disease is a neurodegenerative condition characterized by large intraneuronal aggregates in the brain. It has been hypothesized that these large aggregates may form from smaller soluble protein assemblies, often termed oligomers. ASA-PD, is a new imaging method to generate large-scale α-synuclein oligomer maps in post-mortem human brain tissue.
The VEGFs/VEGFRs system in Alzheimer’s and Parkinson’s diseases: Pathophysiological roles and therapeutic implications
By Emma Sherrell onThis article focuses on the VEGFs/VEGFRs involvement in neurodegenerative diseases by reviewing the current literature on the rather complex VEGFs/VEGFRs contribution to the pathogenic mechanisms of Alzheimer’s (AD) and Parkinson’s disease (PD).
QSBB Digital Pathology Resource
By Emma Sherrell onThis digital pathology resource contains digital whole slide images from Lewy body disorders: Parkinson’s disease, Parkinson’s disease with dementia, dementia with Lewy bodies, and control cases.
