Evaluation of an adapted semi-automated DNA extraction for human salivary shotgun metagenomics
By onThis study demonstrates that the authors’ semi-automated protocol is suitable for shotgun metagenomic analysis.
pCAG-MBP-Foldon-ATG9 (723-839)
By onPlasmid for expression of ATG9 C-terminal tail trimer in mammalian cells.
KAT8 compound inhibition inhibits the initial steps of PINK1-dependant mitophagy
By onThe authors provide additional support for KAT8 inhibition as a regulator of mitophagy and autophagy processes.
Raw tomogram of GFP-a-synuclein inclusion in primary mouse neuron expressing GFP-a-synuclein, seeded with PFFs
By onRaw data associated with DOI: 10.1038/s41467-021-22108-0
Protocol for Data Independent Acquisition – Mass spectrometry analysis – a DIA-based Organelle Proteomics
By onHere the authors provide a detailed protocol for the Data Independent Acquisition (DIA)-based mass spectrometry (MS) data acquisition method for proteomic profiling of the Golgi.
H9 ES AAVS1-NGN2 FAM134C/A/B-/-;TEX264-/-; CCPG1-/-
By onES cells were used to create iNeurons lacking ER-phagy receptors FAM134C, A, B, TEX264, and CCPG1. CRISPR/Cas9 was used to introduce a NEUROG2 construct in the AAVS1 locus. Knockouts included CCPG1, RETREG1, RETREG2, RETREG3, and TEX264.
Proband-NFL Analysis
By onCode used for the manuscript "Combining biomarkers for prognostic modelling of Parkinson’s disease" by Niro Viijaratnam and colleagues.
Purification and Crystallization of ATG9 HDIR-ATG101:ATG13 complex
By onPurification and Crystallization of ATG9 HDIR (828-839) fused ATG101 (1-198):ATG13 (1-197).
MBP Pulldown Assay of ATG9A Truncations
By onProtocol describing MBP Pulldown Assay of ATG9A Truncations.
Lentivirus plasmid: pLV[Exp]-U6>PINK1_P1_Seq3-hPGK>mApple
By onPlasmid: Plasmid vector encoding a sgRNA sequence which targets the human PINK1 promoter for CRISPRi, under a U6 promoter and a mApple fluorescent reporter. Generated by Vectorbuilder in the pLV backbone.
Microbes and Parkinson’s Disease: from associations to mechanisms
By onSeveral microbes, including viruses, bacteria, and fungi, have been associated with an increased risk of PD in humans. Microbial infections can induce similar common pathways that are associated with PD, including systemic inflammatory responses, α-synuclein misfolding, and disruption of mitochondria. PD-associated gene mutations can impact host–microbe interactions, suggesting that even familial forms of PD may be influenced by microbes.
pLenti HsATP13A3 D498N
By onTransfer plasmid for lenti viral vector production, Expresses D498N mutant of Homo sapiens ATP13A3
ggtranscript: an R package for the visualization and interpretation of transcript isoforms using ggplot2
By onggtranscript simplifies visualizing transcript structure with new geoms like range(), intron(), junction(), and junction_label_repel(). It extends ggplot2's flexibility to create informative plots for publication.
Immunoprecipitation of NAP1-GFP
By onThis protocol describes the procedure to perform immunoprecipitation of NAP1-GFP from HAP1 cells.
H9 ES AAVS1-NGN2 FAM134B-/-; PiggyBac-Keima-REEP5
By onES cells modified to lack FAM134B and express Keima-REEP5 ER-phagy flux reporter were created using CRISPR/Cas9. They carry NEUROG2 construct in AAVS1 locus and are derived from human embryonic stem cells at blastocyst stage.
Purification of the PE2 nCas9-RT protein
By onThis protocol describes the process of expressing and purifying the nicking Cas9-MMLV RT fusion protein for prime editing.
Code for splicing-accuracy-manuscript
By onCode used for manuscript: Splicing accuracy varies across human introns, tissues and age
