Raw tomogram of GFP-a-synuclein inclusion in primary mouse neuron expressing GFP-a-synuclein, seeded with MSA aggregates
By onRaw data associated with DOI: 10.1038/s41467-021-22108-0
Visualisation and quantification of dendritic spines in cultured human Medium Spiny Neurons (MSNs)
By onThis protocol describes the visualisation of dendritic spines of human neurons cultured on coverslips in vitro and subsequent quantification using the software Imaris.
Preparing of genomic DNA from in vitro cultured cells
By onThis protocol describes a standard procedure for preparing crude cell lysate which can be further analyzed by PCR.
Regular maintenance of human pluripotent stem cells
By onThis protocol describes the regular maintenance and passaging human iPSCs.
Adapting hPSCs cultured on MEFs to feeder-free system
By onThis protocol describes the procedure of adapting human pluripotent stem cells (hPSCs) to feeder-free culturing conditions using mTeSR-plus or StemFlex
AAV.CAP-Mac analysis code
By onFirst release of AAV.CAP-Mac analysis code and files to generate figures.
Spatial transcriptomics reveals molecular dysfunction associated with Lewy pathology
By onThe results identify neurons vulnerable to Lewy pathology in the PD cortex and identify a conserved signature of molecular dysfunction in both mice and humans.
Time to LiD GWAS dataset
By onCode to perform the study of LiD genetic determinants under CPH regression models and functional annotation analyses
Basal Ganglia Neurons in Healthy and Parkinsonian Primates Generate Recurring Sequences of Spikes
By onData consists of interspike intervals from basal ganglia neurons in awake monkeys, pre- and post-parkinsonian treatment with MPTP. Recordings were during spontaneous spiking, without task involvement.
x4 GBA Plasmids
By onThe below plasmids are deposited and available via Addgene:https://www.addgene.org/Anthony_Schapira/ . These have been used in publication: 10.1093/hmg/ddac233 188580 WT GBA pcDNA3.1 GBA (Homo sapiens) 188581 E326K GBA pcDNA3.1 GBA (Homo sapiens) 188582 L444P GBA pcDNA3.1 GBA (Homo sapiens) 188583 N370S GBA pcDNA3.1 GBA (Homo sapiens)
AAV viral DNA and whole RNA recovery for AAV pool experiments in rhesus macaque
By onThis protocol outlines procedures to extract viral DNA and whole RNA from rhesus macaque tissue that had been treated with AAV in vivo
Lentivirus plasmid: pLV[Exp]-U6>KAT8_P1_Seq1-hPGK>mApple
By onPlasmid: Plasmid vector encoding a sgRNA sequence which targets the human KAT8 promoter for CRISPRi, under a U6 promoter and a mApple fluorescent reporter. Generated by Vectorbuilder in the pLV backbone.
pCAG-MBP-ATG9-D830A/E831A
By onPlasmid for expression of human ATG9-D830A/E831A mutant in mammalian cells
Structural basis for membrane recruitment of ATG16L1 by WIPI2 in Autophagy
By onThe authors showed through structural determination how ATG16L1 and WIPI2 interact and compared the other WIPI proteins showing the variety of mechanisms of membrane recruitment by WIPI proteins.
