Whole-cell Patch-Clamp Recordings from Striatal Cholinergic Interneurons in ex vivo Mouse Brain Slices

By Jeffrey Stedehouder and Stephanie Cragg, PhD
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Output Details

Description
This protocol describes the steps to perform whole-cell patch-clamp recordings of striatal cholinergic interneurons (ChIs) previously labelled with the fluorophore mCherry from acute, ex vivo mouse brain slices. This version contains an additional step to label astrocytes using the dry SR101.
Identifier (DOI)
10.17504/protocols.io.3byl4jo5zlo5/v2
Tags
  • Activity Assay
  • Astrocytes
  • Cholinergic neurons
  • Patch-clamp
Team
Team Cragg
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

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