cDNA clone for bacterial expression of human PPM1H D288A
By onPlasmid for bacterial expression of human PPM1H (D288A); contains TEV protease site for removal of 6His tag.
Proband-NFL Analysis
By onCode used for the manuscript "Combining biomarkers for prognostic modelling of Parkinson’s disease" by Niro Viijaratnam and colleagues.
Protocol for Data Independent Acquisition – Mass spectrometry analysis – a DIA-based Organelle Proteomics
By onHere the authors provide a detailed protocol for the Data Independent Acquisition (DIA)-based mass spectrometry (MS) data acquisition method for proteomic profiling of the Golgi.
H9 ES AAVS1-NGN2 FAM134C/A/B-/-;TEX264-/-; CCPG1-/-
By onES cells were used to create iNeurons lacking ER-phagy receptors FAM134C, A, B, TEX264, and CCPG1. CRISPR/Cas9 was used to introduce a NEUROG2 construct in the AAVS1 locus. Knockouts included CCPG1, RETREG1, RETREG2, RETREG3, and TEX264.
Raw tomogram of GFP-a-synuclein inclusion in primary mouse neuron expressing GFP-a-synuclein, seeded with PFFs
By onRaw data associated with DOI: 10.1038/s41467-021-22108-0
KAT8 compound inhibition inhibits the initial steps of PINK1-dependant mitophagy
By onThe authors provide additional support for KAT8 inhibition as a regulator of mitophagy and autophagy processes.
ggtranscript: an R package for the visualization and interpretation of transcript isoforms using ggplot2
By onggtranscript simplifies visualizing transcript structure with new geoms like range(), intron(), junction(), and junction_label_repel(). It extends ggplot2's flexibility to create informative plots for publication.
Microbes and Parkinson’s Disease: from associations to mechanisms
By onSeveral microbes, including viruses, bacteria, and fungi, have been associated with an increased risk of PD in humans. Microbial infections can induce similar common pathways that are associated with PD, including systemic inflammatory responses, α-synuclein misfolding, and disruption of mitochondria. PD-associated gene mutations can impact host–microbe interactions, suggesting that even familial forms of PD may be influenced by microbes.
pLenti HsATP13A3 D498N
By onTransfer plasmid for lenti viral vector production, Expresses D498N mutant of Homo sapiens ATP13A3
Lentivirus plasmid: pLV[Exp]-U6>PINK1_P1_Seq3-hPGK>mApple
By onPlasmid: Plasmid vector encoding a sgRNA sequence which targets the human PINK1 promoter for CRISPRi, under a U6 promoter and a mApple fluorescent reporter. Generated by Vectorbuilder in the pLV backbone.
Purification and Crystallization of ATG9 HDIR-ATG101:ATG13 complex
By onPurification and Crystallization of ATG9 HDIR (828-839) fused ATG101 (1-198):ATG13 (1-197).
MBP Pulldown Assay of ATG9A Truncations
By onProtocol describing MBP Pulldown Assay of ATG9A Truncations.
H9 ES AAVS1-NGN2 FAM134C/A/B-/-;TEX264-/-
By onES cells were modified using CRISPR/Cas9 to lack ER-phagy receptor genes FAM134C, A, B, and TEX264. NEUROG2 construct was inserted into the AAVS1 locus. Cells were derived from human embryonic stem cells at the blastocyst stage.
Code for splicing-accuracy-manuscript
By onCode used for manuscript: Splicing accuracy varies across human introns, tissues and age
H9 ES AAVS1-NGN2 FAM134B-/-; PiggyBac-Keima-REEP5
By onES cells modified to lack FAM134B and express Keima-REEP5 ER-phagy flux reporter were created using CRISPR/Cas9. They carry NEUROG2 construct in AAVS1 locus and are derived from human embryonic stem cells at blastocyst stage.
Purification of the PE2 nCas9-RT protein
By onThis protocol describes the process of expressing and purifying the nicking Cas9-MMLV RT fusion protein for prime editing.
Fixation and imaging of HeLa cells after mitochondrial depolarization
By onA protocol for the fixation and imaging of HeLa cells after mitochondrial depolarization
