pMAL-c5e-Rubicon RH N821K, T911R, K916R
By Emma Sherrell onBacterial protein expression of Rubicon RH domain mutant.
Homology modeling of Pacer RH domain in SWISS-MODEL
By Emma Sherrell onUsing the Rubicon RH - Rab7 crystal structure as a template (6wcw).
Immunofluorescent imaging and analysis
By Emma Sherrell onFollowing treatment with antibodies/genetic methods of introducing fluorescent tags.
Isothermal titration calorimetry of the Rubicon RH domain and Rab7
By Emma Sherrell on1:1 binding of Rubicon and Rab7.
Stable cell line generation via retrovirus
By Emma Sherrell onProduction of pseudotyped virus an subsequent transduction.
HaloTag autophagy flux assay
By Emma Sherrell onPulse-chaseable method for quantifying autophagy flux.
Expression and purification of RH proteins via MBP affinity tag
By Emma Sherrell onAffinity purification followed by size exclusion chromatography.
Figure 7. Pacer expression promotes formation of WIPI2 puncta upon depolarization [FINAL]
By Emma Sherrell onFigure 7. Pacer expression promotes formation of WIPI2 puncta upon depolarization.
Figure 6: Pacer LC3B imaging [FINAL]
By Emma Sherrell onFigure 6. pRAB7A-dependent function of Pacer in mitophagosome expansion.
Figure 5: Pacer Flux [FINAL]
By Emma Sherrell onFigure 5. Pacer is a pS72 RAB7A-dependent activator of mitophagy.
Figure 4: The Pacer RH domain basic triad controls subcellular localization [FINAL]
By Emma Sherrell onFigure 4. The Pacer RH domain basic triad controls subcellular localization.
Figure 3: Rubicon – Rab7 binding colocalization [FINAL] Creators
By Emma Sherrell onFigure 3. pRAB7A binding preferences dictate subcellular localization.
