Adapting hPSCs cultured on MEFs to feeder-free system
By onThis protocol describes the procedure of adapting human pluripotent stem cells (hPSCs) to feeder-free culturing conditions using mTeSR-plus or StemFlex
Regular maintenance of human pluripotent stem cells
By onThis protocol describes the regular maintenance and passaging human iPSCs.
Preparing of genomic DNA from in vitro cultured cells
By onThis protocol describes a standard procedure for preparing crude cell lysate which can be further analyzed by PCR.
Basal Ganglia Neurons in Healthy and Parkinsonian Primates Generate Recurring Sequences of Spikes
By onData consists of interspike intervals from basal ganglia neurons in awake monkeys, pre- and post-parkinsonian treatment with MPTP. Recordings were during spontaneous spiking, without task involvement.
Time to LiD GWAS dataset
By onCode to perform the study of LiD genetic determinants under CPH regression models and functional annotation analyses
Spatial transcriptomics reveals molecular dysfunction associated with Lewy pathology
By onThe results identify neurons vulnerable to Lewy pathology in the PD cortex and identify a conserved signature of molecular dysfunction in both mice and humans.
pCAG-MBP-ATG9-D830A/E831A
By onPlasmid for expression of human ATG9-D830A/E831A mutant in mammalian cells
Lentivirus plasmid: pLV[Exp]-U6>KAT8_P1_Seq1-hPGK>mApple
By onPlasmid: Plasmid vector encoding a sgRNA sequence which targets the human KAT8 promoter for CRISPRi, under a U6 promoter and a mApple fluorescent reporter. Generated by Vectorbuilder in the pLV backbone.
x4 GBA Plasmids
By onThe below plasmids are deposited and available via Addgene:https://www.addgene.org/Anthony_Schapira/ . These have been used in publication: 10.1093/hmg/ddac233 188580 WT GBA pcDNA3.1 GBA (Homo sapiens) 188581 E326K GBA pcDNA3.1 GBA (Homo sapiens) 188582 L444P GBA pcDNA3.1 GBA (Homo sapiens) 188583 N370S GBA pcDNA3.1 GBA (Homo sapiens)
AAV viral DNA and whole RNA recovery for AAV pool experiments in rhesus macaque
By onThis protocol outlines procedures to extract viral DNA and whole RNA from rhesus macaque tissue that had been treated with AAV in vivo
Catalepsy test (Bar test)
By onThe catalepsy test (bar test) was developed to test motor coordination and motor impairments.
RAB3 phosphorylation by pathogenic LRRK2 impairs trafficking of synaptic vesicle precursors
By onThe authors’ results reveal a mechanism by which pathogenic hyperactive LRRK2 may contribute to the altered synaptic homeostasis associated with characteristic non-motor and cognitive manifestations of PD.
APEX-autophagy cargo receptor proteomics with and without lysosomal damage
By onProteomics data associated with DOI: 10.7554/eLife.72328
