Proteomic data reported in doi.org/10.1073/pnas.2219953120 (Golgi-IP, a tool for multimodal analysis of Golgi molecular content)
By onProteomic data associated with doi.org/10.1073/pnas.2219953120 deposited in ProteomeXchange PRIDE repository with PID: PXD038046.
Leucine-rich repeat kinase 2 at a glance
By onAn overview of current knowledge about LRRK2 function, dysfunction, and links to disease.
Western blotting to detect ATP13A2 and ATP13A3
By onProtocol to detect ATP13A2 and ATP13A3 via Western Blotting.
LRRK2 RCKW Protein Purification
By onProtein purification protocol for tag-less LRRK2RCKW as done by Leschziner and Reck-Peterson Labs. Same protocol can be used to purify LRRK1RCKW as well. Original Protocol by David Snead. Modified by Yu Xuan Lin and Mariusz Matyszewski for publication.
The Parkinson’s disease protein alpha-synuclein is a modulator of processing bodies and mRNA stability
By onThe paper describes the ability of alpha-synuclein to bind to P-bodies, machinery that regulates the expression of our genes through mRNAs. When alpha-synuclein abnormally accumulates, the physiologic structure and functions of the P-body are lost.
Electron microscope sample preparation technique_V2
By onEM sample preparation protocol that is designed for processing cultured cell sample.
Lentivirus production for primary neuron transduction
By onThis protocol is associated with the following preprint, published on February 19th 2022: The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds Itika Saha, Patricia Yuste-Checa, Miguel Da Silva Padilha, Qiang Guo, Roman Körner, Hauke Holthusen, Victoria A. Trinkaus, Irina Dudanova, Rubén Fernández-Busnadiego, Wolfgang Baumeister, David W. Sanders, Saurabh Gautam, Marc I. Diamond, F. Ulrich Hartl, Mark S. Hipp bioRxiv 2022.02.18.481043; doi: https://doi.org/10.1101/2022.02.18.481043
Primary data associated with the manuscript “Genome wide screen reveals Rab12 GTPase as a critical activator of pathogenic LRRK2 kinase” (doi: 10.1101/2023.02.17.529028)
By onPrimary data associated with the manuscript "Genome wide screen reveals Rab12 GTPase as a critical activator of pathogenic LRRK2 kinase"
Combining biomarkers for prognostic modelling of Parkinson’s disease
By onParkinson's disease progression varies among patients. Predicting progression accurately is crucial for clinical trial selection. Blood biomarkers like serum NfL, along with genetic factors (GBA, APOE) can enhance prediction beyond age and phenotype.
DQ-BSA assay
By onThis protocol describes the DQ BSA assay for measuring the lysosomal proteolytic activity.
Plasmid-reprogramming of human fibroblasts
By onThis protocol is part of a collection of protocols for the paper, "Glucocerebrosidase, a Parkinson's disease-associated protein, is imported into mitochondria and regulates complex I assembly and function"
Fecal metagenomic sequencing data for PD patients and controls from Rush University Medical Center
By onFecal metagenomic sequencing data associated with Boktor et al. (2023). This dataset includes samples from the Rush University Medical Center cohort.
H9 ES AAVS1-NGN2 GRN-/-
By onH9 ES cells modified using CRISPR/Cas9 lack GRN gene and express NEUROG2 in AAVS1 safe harbor locus. Derived from human blastocyst, these embryonic stem cells can differentiate into iNeurons.
Quantitative proteomics reveals the selectivity of ubiquitin-binding autophagy receptors in the turnover of damaged lysosomes by lysophagy
By onThe authors used proteomics to develop a quantitative snapshot of the proteins involved in lysophagy. Among the proteins identified, they found that TAX1BP1 and TBK1 are both required for lysophagy.
Gavage – food supplementation for stalled weight gain in mice
By onFeeding protocol for Ndufs2fl/fl mice,
Cell culture, transfection, and imaging
By onThis protocol describes general procedures for culturing HeLa cells, transient transfection, and imaging using an Andor Dragonfly spinning disk confocal system.
