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Differentiation of human medium spiny neurons (MSNs) from induced pluripotent stem cells (iPSCs)
Output Details
Description
This protocol generates human medium spiny neurons (MSNs) from induced human pluripotent stem cells. Incorporating key findings from Telzehkin et.al., 2016 and Arber et.al., 2016, this protocol produces MSNs following the in vivo developmental trajectory of MSN via the subpallium and subsequently, the lateral ganglionic eminence. These neurons expresses canonical markers of striatal projection neurons including glutamic acid decarboxylase as well as co-expression of the dopamine- and cAMP-regulated phosphoprotein, Mr 32 kDa (DARPP-32) and the Coup-TFI interacting protein 2 (CTIP2). They are also functionally active demonstrated by presence of intrinsic voltage-dependent sodium and potassium currents as well as the capacity to fire action potential upon current stimulation.
Identifier (DOI)
10.17504/protocols.io.eq2ly79prlx9/v1