Stereotaxic intracranial virus injection in mice
By onThis protocol outlines steps for conducting stereotaxic brain injections of virus in mice in the Ding lab.
Immunohistology for Mouse Brain Sections
By onThis protocol outlines the general steps for preparing mouse brain tissue for immunostaining and histology analysis in the Ding lab.
Ex Vivo Mouse Brain Slice Whole-Cell Patch-Clamp Electrophysiology
By onThis protocol outlines steps for performing ex vivo slice whole-cell patch-clamp electrophysiology experiments, including voltage clamp and current clamp recordings as well as optogenetic stimulation in the Ding lab.
Stereotaxic Optic Fiber Implantation in Mice
By onThis protocol describes implantation of one or multiple optic fiber cannulae in mouse brains used for optical recording brain activity using fiber photometry or for optogenetic manipulation of neuronal activity in mice performing behavioral tasks.
Head-fixed Lever-pushing Task in Mice
By onThis protocol describes a head-fixed lever-pushing task used to study motor learning and memory in mice suitable for recording neuronal activity concurrently.
Head-fixed Locomotion Task in Mice
By onThis protocol describes a head-fixed locomotion task used to study motor learning in mice suitable for recording neuronal activity concurrently.
Accelerating Rotarod Task in Mice
By onThis protocol describes an accelerating rotarod task used to study motor learning in mice in the Ding lab.
Optogenetic Silencing of Projection Terminals
By onThis protocol describes the combination of protocols and techniques that are employed to optogenetically silence projections from various areas in the brain.
Immunohistochemistry (Brain organoids)
By onProtocol for preparing brain organoid slides and performing immunostainings to analyze protein expression and localization in the 3D model.
Molecularly-guided spatial proteomics captures single-cell identity and heterogeneity of the nervous system
By onThe authors use immunostaining-guided laser capture microdissection coupled with LC-MS to perform single-cell spatial proteomics of neuronal populations from the cortex and substantia nigra in the brain and the myenteric plexus in the periphery.
Source Data and protocols for White, et al 2025- The pyrethroid insecticide deltamethrin disrupts neuropeptide and monoamine signaling pathways in the gastrointestinal tract
By onThe manuscript investigates pyrethroid toxicity in enteroendocrine cells and the gastrointestinal tract. It includes source data, statistical output, qPCR gene expression analysis, RNAseq analysis, Metascape pathway analysis, and key resources.
LINE-1 retrotransposons regulate the exit of human pluripotency and early brain development (Preprint)
By onHominoid-specific L1 retrotransposons are expressed in human stem cells and organoids. Silencing L1s leads to changes in neural differentiation and organoid size, suggesting L1 involvement in central nervous system development.
Data associated with “Structural pathway for PI 3-kinase regulation by VPS15 in autophagy”
By onAll data from the manuscript "Structural pathway for PI 3-kinase regulation by VPS15 in autophagy" All figures can be reproduced from the files in this zenodo upload.
Immunohistochemistry (Histology + Imaging)-Mendelsohn et al 2025
By onThe protocol outlines the steps for preparing mouse brain tissue for immunostaining and imaging from Mendelsohn et al 2025.
In Situ Hybridization -Mendelsohn et al 2025
By onThis protocol outlines the steps for preparing mouse brain tissue for In Situ Hybridization from Mendelsohn et al 2025.
(x6) GBA heterozygous and homozygous mutant fibroblast lines available to share
By onCell lines deposited at ATCC used in a publication (10.1093/hmg/ddac233) include UCL-CTRL001, UCL-CTRL002, UCL-CTRL003, UCL-YCTRL001, UCL-E001K, UCL-N001S, and UCL-N002S, each with specific RRIDs.
EMD-48233
By onPI3KC3-C1 bound to RAB1A. Local refinement of Bara like domains of BECN1 and ATG14, and VPS15 WD40 domain
