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Golgi immunopurification (Golgi-IP) for subcellular metabolite profiling
Output Details
Description
The Golgi is a membrane-bound organelle that is central to protein and lipid processing, sorting and secretion in the cell. Despite its critical cellular function, there has been challenges to quantitatively assess Golgi metabolite profiles. To overcome this hurdle, we developed a rapid harvesting and purification method using immunoprecipitation (Golgi-IP). This protocol provides details for preparing Golgi-IP metabolomics samples.
Identifier (DOI)
10.17504/protocols.io.36wgqj3p3vk5/v1