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Microscopy-based bead protein-protein interaction assay.
Output Details
Description
This protocol describes how to perform microscopy-based bead protein-protein interaction assay with GST- or mCherry-tagged proteins as baits and fluorescently-tagged proteins as preys. The protocol requires to have purified proteins and allows to monitor protein-protein interaction in an equilibrium state. The fluorescent signal can be quantified.
Identifier (DOI)
10.17504/protocols.io.dm6gp3erpvzp/v1