Microscopy-based bead protein-protein interaction assay.

Research Community
Research Community

ASAP supports three programs and serves as a member of multiple consortia to advance research for the benefit of the global Parkinson’s disease community.
Explore
Highlights
Highlights

ASAP shares the impact that our initiative, network, and supported programs have had on providing new insights into Parkinson’s disease. Discover news, interviews, awards, and annual impact.
Explore
Open Science
Open Science

ASAP is dedicated to facilitating a research environment where meaningful collaboration, research-enabling resources, and data sharing provides the answers we need to understand, diagnose, and treat Parkinson’s disease. Discover our open science philosophy.
Explore
Tools & Resources
Tools & Resources

ASAP is committed to developing and openly sharing research outputs, including data, code, protocols, and key lab materials, to help the scientific community build upon existing work.
Explore

Description

This protocol describes how to perform microscopy-based bead protein-protein interaction assay with GST- or mCherry-tagged proteins as baits and fluorescently-tagged proteins as preys. The protocol requires to have purified proteins and allows to monitor protein-protein interaction in an equilibrium state. The fluorescent signal can be quantified.

Identifier (DOI)

10.17504/protocols.io.dm6gp3erpvzp/v1

Tags

Biochemistry
Other
Recombinant

Team

Team Hurley

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

Elisabeth Holzer, MSc

Key Personnel: Team Hurley,

Max Perutz Labs

Microscopy-based bead protein-protein interaction assay.

Output Details

Meet the Authors

Elisabeth Holzer, MSc

Related Research

Damaged mitochondria recruit the effector NEMO to activate NF-κB signaling

GUV assay

In vitro synthesis of PE2 (nCas9-MMLV RT fusion) polyA mRNA using T7 RNA polymerase