Nuclei Isolation and Sorting from Frozen Human Temporal Cortex V2
By onProtocol focuses on isolating and sorting nuclei from frozen human temporal cortex.
The lipid flippase ATP10B enables cellular lipid uptake under stress conditions
By onATP10B mutations are linked to Parkinson's and Lewy body disease. ATP10B acts as a lipid transporter in late endo-/lysosomes, enhancing phosphatidylcholine uptake in cells under stress conditions like rotenone treatment.
GI Transit Assay
By onThis test is used to check for possible signs of constipation and alteration in gut transit.
Seeding nucleofected hPSCs in 96-well plates using limited dilution
By onThis protocol describes a standard procedure for seeding nucleofected human pluripotent stem cells in 96-well plates using limited dilution. This protocol follows nucleofection of hPSCs as described in detail in the collection "Nucleofection (Amaxa) and electroporation (Biorad) of hPSCs;" dx.doi.org/10.17504/protocols.io.b4qnqvve
HeLa ::TMEM192-HA CALCOCO1-/-
By onHeLa ::TMEM192-HA CALCOCO1-/- PubMed=37757899; Population: African American. Characteristics: Using CRISPR/Cas9 TMEM192 was C-terminally tagged on both alleles with a 3xHA epitope (from parent cell line). Knockout cell: Method=CRISPR/Cas9; HGNC; 29306; CALCOCO1. Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18). Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002. NCIt; C27677; Human papillomavirus-related cervical adenocarcinoma NCBI_TaxID=9606; ! Homo sapiens (Human) RRID:CVCL_D1KR ! HeLa::TMEM192-3xHA Female 30Y6M Cancer cell line
Perfusion-fixation procedure for adult rhesus monkeys
By onThe protocol details the perfusion-fixation procedure for rhesus monkeys
Primary data associated with the manuscript “Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55B” (doi: 10.1101/2023.06.07.544051)
By onConfocal microscopy images, CellProfiler Excel files of Pearson's coefficients between TMEM55B/RILPL1 or pRab10/RILPL1 in R1441C MEF or VPS35 MEF cells as seen in doi: 10.1101/2023.06.07.544051.
Behavioral Testing – Open Field and Dyskinesia Scoring
By onThis protocol describes behavioral testing to assess motor deficits in mice. It includes open field locomotor testing and scoring of levodopa-induced dyskinesia.
Pathogenic LRRK2 control of primary cilia and Hedgehog signaling in neurons and astrocytes of mouse brain
By onPathogenic mutations in LRRK2 can cause loss of primary cilia in neurons. The authors show that cilia loss is seen very early in mice harboring the most common LRRK2 mutation.
pCMV-pOTC-GFP
By onPlasmid: Expression of GFP fused with pOTC, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase.
HyDrop-RNA v1.0
By onStep-by-step protocol for performing HyDrop-RNA. The duration of each step assumes an experienced protocol user. For a first-time user, we recommend doubling the expected time for each step.
The non-specific lethal complex regulates genes and pathways genetically linked to Parkinson’s disease
By onHere, the authors sought to identify whether the non-specific lethal complex has potential regulatory relationships with other genes associated with Parkinson's disease in human brain.
pGST2-GST-TEV-NDP52
By onPlasmid: Plasmid for the expression and purification of GST-TEV-NDP52 in E. coli Rosetta pLysS. Internal reference: SMC1586
Investigation_of_mitophagy_in_Hippo_neurons
By onWe developed a method for assessing mitochondrial clearance in primary hippocampal neurons.
Localization of PPM1H phosphatase tunes Parkinson’s disease-linked LRRK2 kinase-mediated Rab GTPase phosphorylation and ciliogenesis
By onThe data support a model in which localization drives PPM1H substrate selection and centriolar PPM1H is critical for regulation of Rab GTPase-regulated ciliogenesis.
