PPM1M, a LRRK2-counteracting, phosphoRab12-preferring phosphatase with potential link to Parkinson’s disease
By onLRRK2 phosphorylates Rab GTPases linked to Parkinson's disease. PPM1M identified as a phosphatase reversing Rab phosphorylation, crucial in LRRK2 pathway. PPM1M mutation found in Parkinson's patients, suggesting a new therapeutic target.
EndoMAP.v1, a Structural Protein Complex Landscape of Human Endosomes
By onEarly endosomes regulate protein fate, sorting plasma membrane proteins for recycling or degradation. Cross-linking and computational analysis were used to create a human endosomal structural interactome, revealing potential regulatory mechanisms.
Phosphatome-wide siRNA screen in 3T3 cells
By onProtocol details a phosphatome-wide siRNA screen in 3T3 cells to identify phosphatases counteracting LRRK2 for pRab12 and pRab10.
Lysosomal GCase (glucocerebrosidase) activity assay
By onMethod to measure lysosomal GBA1 enzyme activity using 4-MUG substrate at low pH to minimize non-lysosomal GCase interference. GBA1 activity higher in purified lysosomes than whole cell extract, confirmed by CBE inhibitor abolishing 4-MUG hydrolysis.
Data upload for Tagless LysoIP method for molecular profiling of lysosomal content in clinical samples
By onSummary: Raw data for the tagless LysoIP manuscript is available.
PPM1M knockout Mouse Embryonic Fibroblasts (mouse)
By onPPM1M knockout mouse embryonic fibroblasts compared to wild-type littermate controls.
Protocol for the production of RAB1A-10xHis in HEK293Gnti cells
By onProtocol for production of RAB1A-10xHis
Preparation of Acute Brain Slices
By onThis protocol describes the steps for preparing acute brain slices.
Segregated basal ganglia output pathways correspond to genetically divergent neuronal subclasses I
By onThis dataset provides a unifying logic for how the developmental specification of diverse SNr neurons relates to the anatomical organization of basal ganglia circuits controlling specialized downstream brain regions.
Single nucleus RNA sequencing of SNr neurons
By onThese findings provide a unifying logic for how the developmental specification of diverse SNr neurons relates to the anatomical organization of basal ganglia circuits controlling specialized downstream brain regions.
pAAVDJ-CAG-FlpX-mKate2.0-N2cG
By onThe pAAV-CAG-fDIO-mKate2.0-N2cG vector was used as a helper virus in conjunction with a rabies virus to label presynaptic inputs to various regions in the brain with focus on SNr neurons.
ATG2A engages Rab1a and ARFGAP1 positive membranes during autophagosome biogenesis
By onATG2A aids in autophagosome formation by localizing to extra-Golgi ARFGAP1 puncta with Rab1 during early stages. Rab1 is crucial for autophagy, and disruptions in autophagosome formation lead to accumulation of ARFGAP1 and Rab1a at ectopic sites.
Soluble Immune Factor Profiles in Blood and CSF Associated with LRRK2 Mutations and Parkinson’s Disease
By onThis preprint explores immune factors in Parkinson's disease linked to LRRK2 mutations. Serum showed elevated SDF-1 alpha and TNF-RII levels in LRRK2 carriers, while CSF had reduced immune markers. PD patients displayed reduced CSF inflammation.
Comparative analysis of AAV serotypes for transduction of olfactory sensory neurons
By onThis study compared the efficacy and selectivity of 11 different AAV serotypes for the efficient and selective transduction of murine olfactory sensory neurons in vivo. The findings inform research approaches and gene therapy based initiatives.
Advances in mitophagy initiation mechanisms
By onThis review will cover recent advances in our understanding of how mitophagy is initiated, and by doing so reveal the mechanistic plasticity of how autophagosome formation can begin.
Stereotaxic Surgery
By onThis protocol describes the steps for performing mouse stereotaxic surgery. It is applicable to intracranial injections of viruses and drugs.
Postsynaptic adaptations in direct pathway muscarinic M4-receptor signaling follow the temporal and regional pattern of dopaminergic degeneration
By onImbalances in dorsal striatum output in Parkinson's disease are driven by dopamine loss and disrupted acetylcholine signaling. These changes occur in response to dopamine loss, affecting M4 receptors in striatal neurons, crucial for PD progression.
6-OHDA mouse model of Parkinson’s disease
By onProtocol for creating a Parkinson's disease mouse model using 6-OHDA involves injecting different doses into the brain to induce varying levels of dopaminergic damage.
Levodopa treatment for low-dose 6-OHDA-treated mice
By onThis protocol describes a one-week levodopa treatment for mice partially depleted of dopamine.
Electrophysiology and 2-photon imaging of Ca+2-transients
By onProtocol for imaging dendritic calcium transients using 2-photon microscopy with a Ti:Sapphire laser. Fluorescence collected through objective, dichroic mirror, and filters, then detected by a GaAsP PMT. Output digitized for analysis.
