mRNA purification by translating ribosome affinity purification (TRAP) of DAT-expressing cells (dopaminergic neurons) in mouse ventral midbrain
By savannah onThis protocol describes the capture of eGFP-L10a-tagged ribosomes and mRNA from DAT-expressing cells (dopaminergic neurons) in mouse ventral midbrain.
Mitochondrial isolation protocol
By savannah onThis protocol describes how to isolate crude mitochondrial fractions from HeLa cells.
Sleep-wake recording in the Turek Laboratory
By savannah onSurgery and recording protocol for measuring sleep-wake behavior in mice for Turek Laboratory.
SDS-PAGE and Western blot analysis
By savannah onThis protocol describes SDS-PAGE and Western blot analysis.
Rapalog-induced chemical dimerization experiments
By savannah onThis protocol describes Rapalog-induced chemical dimerization experiments.
Expression and purification of mCherry-OPTN
By savannah onThis protocol describes the purification of mCherry-OPTN.
Induction of non-selective bulk autophagy
By savannah onThis protocol describes how to induce bulk (non-selective) autophagy in HeLa cells through nutrient starvation.
HeLa S3 AZI2/TBKBP1 DKO clone 14
By savannah onCell line from an African American Population. Knockout cell: Method=CRISPR/Cas9; HGNC; 24002; AZI2. Knockout cell: Method=CRISPR/Cas9; HGNC; 30140; TBKBP1. Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18). Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
HeLa S3 penta KO-AZI2/TBKBP1 DKO clone 20
By savannah onCell line from an African American Population. Knockout cell: Method=CRISPR/Cas9; HGNC; 29912; CALCOCO2. Knockout cell: Method=CRISPR/Cas9; HGNC; 24002; AZI2. Knockout cell: Method=CRISPR/Cas9; HGNC; 6746; NBR1. Knockout cell: Method=TALEN; HGNC; 17142; OPTN. Knockout cell: Method=CRISPR/Cas9; HGNC; 11280; SQSTM1. Knockout cell: Method=CRISPR/Cas9; HGNC; 11575; TAX1BP1. Knockout cell: Method=CRISPR/Cas9; HGNC; 30140; TBKBP1.
HeLa S3 penta KO-AZI2/TBKBP1 DKO clone 26
By savannah onCell line from an African American Population. Knockout cell: Method=CRISPR/Cas9; HGNC; 29912; CALCOCO2. Knockout cell: Method=CRISPR/Cas9; HGNC; 24002; AZI2. Knockout cell: Method=CRISPR/Cas9; HGNC; 6746; NBR1. Knockout cell: Method=TALEN; HGNC; 17142; OPTN. Knockout cell: Method=CRISPR/Cas9; HGNC; 11280; SQSTM1. Knockout cell: Method=CRISPR/Cas9; HGNC; 11575; TAX1BP1. Knockout cell: Method=CRISPR/Cas9; HGNC; 30140; TBKBP1.
pBMN-HA-NAP1-L226Q/L233Q (delta-TBK1)
By savannah onPlasmid: For mammalian expression of NAP1 (delta-TBK1).
pHAGE-FKBP-GFP-NAP1
By savannah onPlasmid: Stably express GFP-tagged NAP1 for CID induced mitophagy.
pHAGE-FKBP-GFP-NAP1-S37K/A44E (delta-NDP52)
By savannah onPlasmid: Stably express GFP-tagged NAP1 (delta-NDP52) for CID induced mitophagy.
pHAGE-FKBP-GFP-NAP1-I11S/L12S (delta-FIP200)
By savannah onPlasmid: Stably express GFP-tagged NAP1 (delta-FIP200) for CID induced mitophagy.
pHAGE-FKBP-GFP-NAP1-L226Q/L233Q (delta-TBK1)
By savannah onPlasmid: Stably express GFP-tagged NAP1 (delta-TBK1) for CID induced mitophagy.
pHAGE-FKBP-GFP-OPTN
By savannah onPlasmid: Stably express GFP-tagged OPTN for CID induced mitophagy.
