ImageJ FIJI code to semi-automatize the morphological analysis of microglia in histological sections
By onImageJ FIJI code to semi-automatize the morphological analysis of microglia in histological sections.
Immunohistochemistry of AAV-treated tissues
By onOutlines procedures to perform immunohistochemistry on tissue that had been treated with AAV.
Passaging of hPSCs grown on MEFs
By onThis protocol describes the standard procedure of using collagenase to passage human pluripotent stem cells (hPSCs) on inactivated mouse embryonic fibroblasts (MEFs).
Unilateral intranigral AAV alpha synuclein mouse model
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Intracellular Cytokine (ICS) Staining Protocol
By onThis protocol details about intracellular cytokine (ICS) staining.
H9 ES AAVS1-NGN2 FAM134C/A/B-/-; PiggyBac-Keima-REEP5
By onES cells were modified to create iNeurons lacking FAM134C, A, B receptors and expressing Keima-REEP5 ER-phagy flux reporter. CRISPR/Cas9 was used to introduce NEUROG2 construct in AAVS1 locus and knockout RETREG1, 2, 3 genes.
Fluorescent Immunolabelling for endogenous ChAT in mice striatum
By onThis immunohistochemistry protocol has been designed and adjusted specifically for ChAT immunolabelling in the mice striatum.
Immunofluorescent labeling of cultured cells
By onThis protocol describes the process of Paraformaldehyde fixation and immunofluorescent-labeling of cultured cells.
A feed-forward pathway drives LRRK2 kinase membrane recruitment and activation
By onLRRK2 interacts with Rab8A and Rab10 at specific binding sites, enhancing LRRK2 kinase activity on membranes. This feed-forward pathway regulates LRRK2 activation and substrate phosphorylation.
cDNA clones for expression of GolgiTag (TMEM115) in mammalian cells
By onPlasmids for expression of TMEM115 (GolgiTag) in human and mouse cells.
Evaluation of mtKeima foci in induced neurons (iNeurons)
By onAssociated with preprint: https://www.biorxiv.org/content/10.1101/2022.11.02.514817v1
Piggybac-mediated stable expression of NGN2 in iPSCs for differentiation into excitatory glutamatergic neurons V.2
By onThe authors adapted a previously described method for employing Piggybac transfection to stably express doxycycline-inducible NGN2 in human iPSCs.
Electrophysiological recording from a chronic chamber-implanted non-human primate
By onThis protocol describes the preparation and insertion of extracellular recording electrodes into intracranial targets of a non-human primate implanted with a chronic recording chamber.
Pluripotency markers staining
By onThis protocol describes the standard procedure for staining pluripotency markers, e.g., OCT4, SSEA4, alkaline phosphatase, and etc. on human pluripotent stem cells (hPSCs).
Multiscale model of primary motor cortex circuits predicts in vivo cell type-specific, behavioral state-dependent dynamics
By onA detailed multiscale model of mouse primary motor cortex accurately predicted responses to behavioral states and manipulations, aiding in understanding cortical function at different scales.
Freezing of mouse embryonic fibroblasts (MEFs) for hPSC cultures
By onThis protocol describes the freezing of mouse embryonic fibroblasts (MEFs), which can later be used as feeder cells for human pluripotent stem cell (hPSC) culture.
Subcellular and regional localization of mRNA translation in midbrain dopamine neurons–DropSeqPipeline8
By onThis is a data processing pipeline for analyzing microwell- or DropSeq-like scRNA-seq data. It can also be used for analyzing pooled plate-based scRNA-seq
Genotyping mice from ear clips
By onThis protocol describes the genotyping procedure from ear clip samples. This includes a general PCR protocol for primers with annealing temperatures in the range 55-70 degrees C. For other primers, the thermal cycling should be adjusted.
Ectopic receptor & endosome size and count image analysis and quantification
By onThis protocol is to help with the imaging analysis of ectopic receptor localization and endosome size.
