Immunocytochemistry for CASR in iPSc-derived dopaminergic neurons
By Julia Julia Leonard onCASR immunocytochemistry protocol to accompany Kilfeather, Khoo et al., 2023: Single cell spatial transcriptomic and translatomic profiling of dopaminergic neurons in health, aging, and disease.
Assessing autophagy using the HaloTag-LC3B cleavage assay
By Emma Sherrell onAssay to detect HaloTag-LC3B during starvation autophagy based on https://doi.org/10.7554/eLife.78923.
DAT-TRAP Protocol
By Julia Julia Leonard onThis protocol describes the capture of eGFP-L10a-tagged ribosomes and mRNA from DAT-expressing cells in mouse ventral midbrain.
Fast-scan cyclic voltammetry to assess dopamine release in ex vivo mouse brain slices
By Julia Julia Leonard onThis protocol is to assess whether a drug changes the dopamine concentration released following a single pulse (1p) electrical stimulation.
Three-step docking by WIPI2, ATG16L1 and ATG3 delivers LC3 to the phagophore: Molecular dynamics simulation dat
By Emma Sherrell onAtomistic molecular dynamics simulation data set accompanying manuscript "Three-step docking by WIPI2, ATG16L1 and ATG3 delivers LC3 to the phagophore."
Peripheral MC1R activation modulates immune responses and is neuroprotective in a mouse model of Parkinson’s disease
By Julia Julia Leonard onPreprint: The present study investigates the impact of NDP-MSH, a synthetic melanocortin receptor (MCR) agonist that does not cross BBB, on the immune system and the nigrostriatal dopaminergic system in mouse model of PD.
Raw band intensity values for ATG3 WT or Mutants in vitro LC3 lipidation
By Emma Sherrell onRaw band intensity values used for the quantification of in vitro LC3 lipidation results (Fig4C) in Three-step docking by WIPI2, ATG16L1 and ATG3 delivers LC3 to the phagophore.
Raw band intensity values for Halo-LC3B
By Emma Sherrell onRaw band intensity values used for the quantification of percentage autophagy levels.
Whole mount dissection and staining of enteric nervous system
By Julia Julia Leonard onThis protocol details whole mount dissection and staining of the enteric nervous system.
Protocol for in vivo electrophysiological and optogenetic manipulation of basal ganglia neurons in awake head-fixed mice
By Julia Julia Leonard onStep by step procedure for in vivo electrophysiological and optogenetic manipulation of basal ganglia neurons in awake head-fixed mice.
Headplate surgery protocol for in vivo electrophysiological and optogenetic manipulation of basal ganglia neurons in awake head-fixed mice
By Julia Julia Leonard onStep by step protocol for headplate surgery for in vivo electrophysiological and optogenetic manipulation of basal ganglia neurons in awake mice.
Stereotaxic injection of viral vectors
By Julia Julia Leonard onStereotaxic injection of viral vectors.
Protocol for ex vivo patch clamping
By Julia Julia Leonard onProtocol for ex vivo patch clamping Protocol for ex vivo patch clamping.
Brain processing, slicing, and immunohistochemistry protocol
By Julia Julia Leonard onThis protocol is a step by step procedure from collecting brain samples to immunohistochemical staining and mounting brain slices.
Handling and behavioral training protocol for in vivo electrophysiological and optogenetic manipulation of basal ganglia neurons in awake head-fixed mice
By Julia Julia Leonard onThis protocol details the habituation of animals and behavioral training of animals for in vivo electrophysiology or optogenetic experiments.
Confocal imaging on Nikon AXR confocal microscope
By Julia Julia Leonard onThis protocol provides a step by step protocol to acquire images on Nikon AXR confocal set-up.
Immunofluorescence staining
By Julia Julia Leonard onThe protocol describes immunofluorescence staining on brain sections.
Automated immunohistochemistry staining
By Julia Julia Leonard onThe protocols describe the steps for automated immunohistochemistry staining using the Ventana Discovery® XT Immunostainer.
Luminescent conjugated oligothiophenes (LCO) staining
By Julia Julia Leonard onThe protocol describes Luminescent Conjugated Oligothiophenes staining on brain sections.