Fluorescence in situ hybridization (FISH)
By onFluorescence in situ hybridization (FISH) in Conserved and cell type-specific transcriptional responses to IFN-γ in the ventral midbrain
Multiscale model of primary motor cortex circuits predicts in vivo cell type-specific, behavioral state-dependent dynamics
By onThe authors developed a multiscale model of mouse primary motor cortex with over 10,000 neurons and 30 million synapses. The model accurately predicted cell type-specific responses associated with behavioral states and experimental manipulations
pAC150- TEX264(deltaLIR, F273A)-GFP
By onSummary: A Piggybac vector is used to express a TEX264-GFP reporter with a delta LIR point mutant, facilitating genetic research applications.
WIPI2d Expression and purification
By onProtocol for WIPI2d expression and purification from HEK cells.
FACS-based enrichment of transfected hPSCs
By onThis protocol describes the procedure FACS-based enrichment of transfected human pluripotent stem cells (hPSCs).
Tomogram data of TauRD-Y forms amyloid-like aggregates in cells
By onTomograms: The tomograms shown in Fig. 1d and Fig. 3c are available in the EMDB https://www.ebi.ac.uk/emdb/ through the following information: EMD ID: EMD-13739 (Fig. 1d) and EMD-13740 (Fig. 3c)
KOLF2.1 iPSCs AAVS1-NGN2 ATG12-/-
By onSummary: iPSCs lacking autophagy factor ATG12 used to generate iNeurons from KOLF2.1 iPSCs with NEUROG2 introduced using CRISPR/Cas9 in AAVS1 safe harbor locus.
pLenti HsATP13A3 WT
By onTransfer plasmid for lenti viral vector production, expresses wild-type Homo sapiens ATP13A3
pCAG-GST-ATG9 (828-839)-ATG101 (1-198)
By onPlasmid: To make ATG101/ATG13-HORMA and ATG9 C-terminal tail fusion complex for expression in mammalian cells.
Untargeted metabolomics analysis for Golgi immunopurification (Golgi-IP)
By onThis protocol provides details for analyzing GolgiIP metabolomics samples using liquid chromatography mass spectrometry (LC-MS) for polar metabolite profiling.
Radiolabeled polyamine uptake in cells
By onThis protocol provides a technique to determine the radiolabeled polyamine uptake capacity in cells, via the acquisition of disintegrations per minute (DPM) using a Liquid Scintillation Counter.
TNF-NF-κB-p53 axis restricts in vivo survival of hPSC-derived dopamine neurons
By onThe ASAP-related findings are the DA neuron purification strategy developed (CD49e-/CD184+) developed and validated in this manuscript, which is suitable for PD-iPSC based disease modeling and DA-neuron related CRISPR screens from our consortium.
Rapid iPSC inclusionopathy models shed light on formation, consequence, and molecular subtype of α-synuclein inclusions
By onThe authors developed an iPSC toolbox utilizing piggyBac-based or targeted transgenes to rapidly induce CNS cells with concomitant expression of aggregation-prone proteins.
Probedesign pipeline for the inhouse generation of seqFISH probes
By onThe probe design pipeline works by inputting the transcript id's for probes to be designed. The pipeline will output the designed probes in fasta format and a csv file with the results of all filtering steps.
Astrocyte isolation and culturing
By onThis protocol details steps for preparing an astrocyte monoculture from mouse pups. From dissection to plated samples ready to be transfected, treated, lysed, or imaged, the protocol takes 20-25 days.
Primary data associated with the manuscript “Genome-wide screen reveals Rab12 GTPase as 2 a critical activator of Parkinson’s disease-linked LRRK2 kinase” (2/3)
By onPrimary data associated with the manuscript, "Genome-wide screen reveals Rab12 GTPase as 2 a critical activator of Parkinson’s disease-linked LRRK2 kinase."
