Radiolabeled polyamine uptake in cells

By Marine Houdou, PhD, Nathalie Jacobs, MSc and Peter Vangheluwe, PhD
View Published Protocol

Output Details

Description

This protocol provides a technique to determine the radiolabeled polyamine uptake capacity in cells, via the acquisition of disintegrations per minute (DPM) using a Liquid Scintillation Counter.

Identifier (DOI)
10.17504/protocols.io.yxmvm2x85g3p/v1
Tags
  • Polyamine
Team
Team Vangheluwe
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

Related Research

ATP13A2-mediated endo-lysosomal polyamine export counters mitochondrial oxidative stress

Recessive loss-of-function mutations in ATP13A2 (PARK9) are associated with a spectrum of neurodegenerative disorders, including Parkinson’s disease (PD). We recently revealed that the late endo-lysosomal transporter ATP13A2 pumps polyamines like…

View Article

Functional characterization of ATP13A2 variants associated with distinct neurodegenerative disorders

ATP13A2 is a late endolysosomal transporter that exports the polyamines spermine and spermidine from the organellar lumen to the cytosol. Loss-of-function variants in ATP13A2 are causative for Kufor-Rakeb syndrome (KRS, a recessive juvenile-onset…

View Article

P5B-ATPases in the mammalian polyamine transport system and their role in disease

Polyamines (PAs) are physiologically relevant molecules that are ubiquitous in all organisms. The vitality of PAs to the healthy functioning of a cell is due to their polycationic nature causing them to interact with a vast plethora of cellular…

View Article
View More Outputs