FACS-based enrichment of transfected hPSCs
By onThis protocol describes the procedure FACS-based enrichment of transfected human pluripotent stem cells (hPSCs).
KOLF2.1 iPSCs AAVS1-NGN2 ATG12-/-
By onSummary: iPSCs lacking autophagy factor ATG12 used to generate iNeurons from KOLF2.1 iPSCs with NEUROG2 introduced using CRISPR/Cas9 in AAVS1 safe harbor locus.
pCAG-GST-ATG9 (828-839)-ATG101 (1-198)
By onPlasmid: To make ATG101/ATG13-HORMA and ATG9 C-terminal tail fusion complex for expression in mammalian cells.
Radiolabeled polyamine uptake in cells
By onThis protocol provides a technique to determine the radiolabeled polyamine uptake capacity in cells, via the acquisition of disintegrations per minute (DPM) using a Liquid Scintillation Counter.
Untargeted metabolomics analysis for Golgi immunopurification (Golgi-IP)
By onThis protocol provides details for analyzing GolgiIP metabolomics samples using liquid chromatography mass spectrometry (LC-MS) for polar metabolite profiling.
TNF-NF-κB-p53 axis restricts in vivo survival of hPSC-derived dopamine neurons
By onThe ASAP-related findings are the DA neuron purification strategy developed (CD49e-/CD184+) developed and validated in this manuscript, which is suitable for PD-iPSC based disease modeling and DA-neuron related CRISPR screens from our consortium.
Rapid iPSC inclusionopathy models shed light on formation, consequence, and molecular subtype of α-synuclein inclusions
By onThe authors developed an iPSC toolbox utilizing piggyBac-based or targeted transgenes to rapidly induce CNS cells with concomitant expression of aggregation-prone proteins.
Probedesign pipeline for the inhouse generation of seqFISH probes
By onThe probe design pipeline works by inputting the transcript id's for probes to be designed. The pipeline will output the designed probes in fasta format and a csv file with the results of all filtering steps.
Astrocyte isolation and culturing
By onThis protocol details steps for preparing an astrocyte monoculture from mouse pups. From dissection to plated samples ready to be transfected, treated, lysed, or imaged, the protocol takes 20-25 days.
Fluorescently labeled polyamine uptake (via Flow Cytometry)
By onAssess polyamine uptake capacities of a specific cell line after incubation with fluorescently labeled polyamines and mean fluorescence intensity acquisition via flow cytometry.
Immunostaining Mouse Brain Tissue or Neuronal Cultures
By onThis protocol describes immunohistochemical and immunoctyochemical methods to analyze protein expression in mouse brain. In the protocol, we describe the fixation of tissue or cultured neurons, the immunostaining procedure, and collecting images for analysis.
Primary data associated with the manuscript “Genome-wide screen reveals Rab12 GTPase as 2 a critical activator of Parkinson’s disease-linked LRRK2 kinase” (2/3)
By onPrimary data associated with the manuscript, "Genome-wide screen reveals Rab12 GTPase as 2 a critical activator of Parkinson’s disease-linked LRRK2 kinase."
R code for paper Phenotype of GBA1 variants in individuals with and without Parkinson disease: the RAPSODI study
By onThis is the R code used to produce the results described in the paper "Phenotype of GBA1 variants in individuals with and without Parkinson’s disease: The RAPSODI study"
Microscopy-based bead protein-protein interaction assay.
By onProtocol for microscopy-based bead protein-protein interaction assay using GST- or mCherry-tagged baits and fluorescently-tagged preys. Requires purified proteins, monitors interactions in equilibrium, and allows quantification of fluorescent signal.
Golgi immunopurification (Golgi-IP) for subcellular metabolite profiling
By onThis protocol provides details for preparing Golgi-IP metabolomics samples.
