Multiple sequence alignment
By Emma Sherrell onProcedure for multiple sequence alignment used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function."
pLP201_pCCL_EFSp_GreenLantern_WPRE
By Emma Sherrell onpLP201_pCCL_EFSp_GreenLantern_WPRE fluorescent reporter plasmid.
pLP202_pCCL_EFSp_mScarlet_WPRE
By Emma Sherrell onpLP202_pCCL_EFSp_mScarlet_WPRE fluorescent reporter plasmid.
Quantification of p62-positive inclusions
By Emma Sherrell onQuantification of p62-positive inclusions in the rodent brain.
Magnetic resonance imaging (MRI) of mouse brain
By Emma Sherrell onMagnetic resonance imaging (MRI) of mouse brain.
Passive avoidance (step-down test) for mice
By Emma Sherrell onPassive avoidance (step-down test) for mice.
Formalin fixed Paraffin Embedded (FFPE) Tissue preparation for digital pathology quantification using QuPath
By Emma Sherrell onQuPath is an open-source digital pathology analysis software. Here, it is used to quantify various misfolded proteins across anatomical regions. This protocol is appropriate for both human and mouse tissue, and will outline the methods of tissue preparation, digitisation, segmentation, and importing images into QuPath.
Habituation and dishabituation (olfaction test)
By Emma Sherrell onHabituation and dishabituation (olfaction test) for mice.
Raw data associated with each Figure and Supplementary Figures from “Novel green fluorescent polyamines to analyze ATP13A2 and ATP13A3 activity in the mammalian polyamine transport system”
By Emma Sherrell onRaw data associated with each Figure and Supplementary Figures from "Novel green fluorescent polyamines to analyze ATP13A2 and ATP13A3 activity in the mammalian polyamine transport system."
Development and characterization of a non-human primate model of disseminated synucleinopathy
By savannah onIn this study, the performance and biodistribution of the retrogradely-spreading AAV9-SynA53T vector was evaluated in the NHP brain. Conducted intraparenchymal deliveries of viral suspensions in the left putamen gave rise to a disseminated synucleinopathy in a circuit-specific basis.
Neuromelanin accumulation drives endogenous synucleinopathy in non-human primates
By savannah onEvidence has provided showing that intracellular aggregation of endogenous alpha-synuclein is triggered by NMel accumulation, therefore any therapeutic approach intended to decrease NMel levels may provide appealing choices for the successful implementation of novel PD therapeutics.
Polyamines in Parkinson’s Disease: Balancing Between Neurotoxicity and Neuroprotection
By Emma Sherrell onPublished: The authors formulate outstanding research questions regarding the role of polyamines in PD, their potential as PD biomarkers, and possible therapeutic strategies for PD targeting polyamine homeostasis.
ImageJ FIJI code to semi-automatize the morphological analysis of microglia in histological sections
By savannah onImageJ FIJI code to semi-automatize the morphological analysis of microglia in histological sections.