Collaborative Research Network Archive

The authors propose the use of LPS as a positive control for microglia activation assays (e.g., phagocytosis assay, cytokine production, and secretion), and as a phenotyping tool to investigate disease-mutated microglia by comparing to disease-relevant stimuli.

Here, the protocol describes a highly efficient method for differentiating monocytes/macrophages from hiPSC.

Here, the authors present a protocol for quantitative immunostaining of intracellular accessible cholesterol using ALOD4-mNeon in human fibroblasts.

Here, the authors describe an algorithm for segmenting fluorescently-labeled cilia using SSTR3 as a ciliary marker.

Here, the authors describe a protocol for RNA extraction, cDNA synthesis, and quantitative PCR from NIH-3T3 fibroblasts. This method has also been used with mouse embryonic fibroblasts.

Here, the authors present a CellProfiler (PMID: 34507520) software pipeline to quantify the density and intensity of dopaminergic processes in the mouse striatum. The dopaminergic processes in the striatum are stained using an anti-tyrosine hydroxylase antibody and the same sections are also stained using an antibody that recognizes total neuronal NeuN (neuronal nuclear antigen biomarker) for staining normalization.

Culturing of iPSC lines in essential 8 medium, thawing, and passaging techniques.

This protocol describes the authors' method for the differentiation of human floor plate neural progenitor cells into human midbrain-dopaminergic neurons. This protocol has been developed using a combination of several published protocols.

The intent of this Standard Operating Procedure (SOP) is to describe procedures for mouse spinal cord injection surgery.