CRISPR/Cas9 generation of knock-out iPSCs

By Dan Dou, Alexander Boecker, MD and Erika Holzbaur, PhD
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Output Details

Description
This protocol describes RNP-based CRISPR/Cas9 gene-editing to generate knock-out iPSCs. iPSCs are transfected with sgRNA, recombinant Cas9, and GFP using Lipofectamine Stem. After FACS sorting to enrich for successfully transfected cells, individual clones are picked and expanded for further analysis.
Identifier (DOI)
10.17504/protocols.io.36wgqj48yvk5/v1
Tags
  • Cas9
  • CRISPR
  • iPSCs (Induced pluripotent stem cells)
Team
Team Hurley
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

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