H9 ES AAVS1-NGN2 FLAG-EEA1

ES cells for making iNeurons that express FLAG-tagged EEA1 from the endogenous gene for Endo-IPs H9 ES AAVS1-NGN2 3xFLAG-EEA1 Characteristics: Using CRISPR/Cas9 a TRE3G promoter driven NEUROG2 construct was introduced in the AAVS1 safe harbor locus. Characteristics: Using CRISPR/Cas9 EAA1 was N-terminally tagged on both alleles with a 3xFLAG tag (Direct_author_submission). Transfected with: HGNC; 13805; NEUROG2. Miscellaneous: Cell line information from personal communication of Harper J.W. Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. NCBI_TaxID=9606; ! Homo sapiens (Human) RRID:CVCL_C4EK ! H9 AAVS1-TRE3G-NGN2 Female Blastocyst stage Embryonic stem cell