Single cell analysis of iPSC-derived midbrain organoids

By Pascale Baden, Maria Jose Perez Jimenez, PhD, Stefanie Kalb, Hariam Raji, María Illescas, Claudio Giuliano, Federico Bertoli, MD, Alessandra Calogero, Graziella Cappelletti, Kathrin Brockmann, Thomas Gasser, Anthony Schapira, PhD, Cristina Ugalde and Michela Deleidi, MD, PhD
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Output Details

Description

The following script was used for analysis of gene corrected (GC) versus GBA1 mutant (MUT) midbrain organoids. The purpose was to combine, filter, integrate, and identify clusters and differentially expressed genes sets. This is part of a Collection of protocols (dx.doi.org/10.17504/protocols.io.8epv593dng1b/v1) for the paper "Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function" (https://doi.org/10.21203/rs.3.rs-1521848/v1)

Identifier (DOI)
10.5281/zenodo.6475864
Tags
  • Midbrains
  • Organoid
  • scRNAseq (Single-cell RNA-seq)
  • Sequencing
Team
Team Schapira
Program
Collaborative Research Network
Theme
Neuro-Immune Interactions

Meet the Authors

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    Pascale Baden

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    Maria Jose Perez Jimenez, PhD

    Key Personnel: Team Schapira, 

    German Center for Neurodegenerative Diseases

  • User avatar fallback logo

    Stefanie Kalb

  • User avatar fallback logo

    Hariam Raji

  • User avatar fallback logo

    María Illescas

  • User avatar fallback logo

    Claudio Giuliano

  • Federico Bertoli, MD

    Key Personnel: Team Schapira, 

    German Center for Neurodegenerative Diseases

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    Alessandra Calogero

  • User avatar fallback logo

    Graziella Cappelletti

  • User avatar fallback logo

    Kathrin Brockmann

  • User avatar fallback logo

    Thomas Gasser

  • Anthony Schapira, PhD

    Lead PI (Core Leadership): Team Schapira, 

    University College London

  • User avatar fallback logo

    Cristina Ugalde

  • Michela Deleidi, MD, PhD

    Co-PI (Core Leadership): Team Schapira, 

    Institut Imagine

Related Research

Midbrain organoid differentiation in spinner flasks

Midbrain differentiation protocol using spinner flasks

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Collection of protocols for paper: “Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function”

This is a collection of protocols used in a recent preprint by the Deleidi Lab, Team Schapira. You can access pre-print at https://doi.org/10.21203/rs.3.rs-1521848/v1

View Protocol

Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function

Raw data files used for the manuscript "Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function" https://www.researchsquare.com/article/rs-1521848/v1

View Dataset
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