Collection of protocols for paper: “Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function”

By Michela Deleidi, MD, PhD, Maria Jose Perez Jimenez, PhD, Hariam Raji, Federico Bertoli, MD and Pascale Baden
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Output Details

Description

This is a collection of protocols used in a recent pre-print by the Deleidi Lab, Team Schapira. You can access pre-print at https://doi.org/10.21203/rs.3.rs-1521848/v1. This is relevant to the pre-print publication https://hub.asap.science/shared-research/e4c58c5f-d833-4ed3-81a8-e3ee96094799/edit

Identifier (DOI)
10.17504/protocols.io.8epv593dng1b/v1
Tags
  • Differentiation
  • GCase activity
  • Human
  • In Vitro
  • iPSC
  • Midbrains
  • Organoid
  • Plasmids
  • Single-cell analysis
Team
Team Schapira
Program
Collaborative Research Network
Theme
Neuro-Immune Interactions

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Related Research

Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function

Raw data files used for the manuscript "Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function" https://www.researchsquare.com/article/rs-1521848/v1

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Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism

Mutations in GBA1, the gene encoding the lysosomal enzyme β-glucocerebrosidase (GCase), which cause Gaucher’s disease, are the most frequent genetic risk factor for Parkinson’s disease (PD). Here, we employ global proteomic and single-cell…

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Differentiation NPCs to Dopaminergic/Midbrain Neurons

This protocol details methods for differentiation of NPCs to Dopaminergic/Midbrain Neurons. This protocol is part of a Collection of protocols (dx.doi.org/10.17504/protocols.io.8epv593dng1b/v1) for the paper “Glucocerebrosidase, a Parkinson´s…

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