Mitophagy induction using Difereprone
By Emma Sherrell onMitophagy induction in HeLa cells using Difereprone (DFP).
Mitophagy induction using Oligomycin/Antimycin A
By Emma Sherrell onMitophagy induction in HeLa cells using Oligomycin/Antimycin A.
Generation of stable cell lines using viral infection
By Emma Sherrell onProtocol for generation and precipitation of retrovirus, and infection of HeLa cells to generate stable cell lines.
Reduction of a-synuclein aggregates by PIKfyve inhibition via TFEB-mediated lysosomal biogenesis in a Parkinson’s disease model
By Emma Sherrell onPreprint: In the present study, the authors exploited the SH-SY5Y cell model overexpressing a pro-aggregation form of alpha-synuclein to investigate the efficacy of PIKfyve-mediated lysosomal biogenesis, through TFEB, as a potential target for Parkinson's therapy.
Feacal metagenomic data related to “Evaluation of an Adapted Semi-Automated DNA Extraction for Human Salivary Shotgun Metagenomics”
By Emma Sherrell onThis study demonstrates that the authors' semi-automated protocol is suitable for shotgun metagenomic analysis, by significantly producing higher DNA fragment sizes while allowing for improved sample treatment logistics with reduced technical variability and without compromising the structure of the oral microbiome.
Salivary collection participant instructions using OMNIgene ORAL OM 501 device
By Emma Sherrell onSalivary collection participant instructions using OMNIgene ORAL OM 501 device.
R scripts for analysis of publication “Sex distribution of GBA1 Variants Carriers with Dementia with Lewy Bodies and Parkinson Disease”
By Emma Sherrell onR scripts for analysis of publication "Sex distribution of GBA1 variant carriers with dementia with Lewy Bodies and Parkinson's disease."
Biochemical consequences of glucocerebrosidase 1 mutations in Parkinson’s disease
By Emma Sherrell onPerspective on the biochemical consequences of glucocerebrosidase 1 mutations in Parkinson’s disease.
Lentiviral vector plasmid for overexpression of ATP10B (E993A)
By Emma Sherrell onPlasmid: Lentiviral vector plasmid for overexpression of ATP10B (E993A) validated for use in cell culture.
Lentiviral vector plasmid for overexpression of ATP10B (I1038T)
By Emma Sherrell onPlasmid: Lentiviral vector plasmid for overexpression of ATP10B (I1038T) validated for use in cell culture.
Lentiviral vector plasmid for knock down of ATP10B (miR5 Hs)
By Emma Sherrell onPlasmid: Lentiviral vector plasmid for knock down of ATP10B (miR5, Hs) validated for use in cell culture.
Dopaminergic denervation and associated MRI microstructural changes in the nigrostriatal projection in early Parkinson’s disease patients
By Emma Sherrell onThe asymmetry between striatal and SNc changes for both dopaminergic depletion and microstructural degeneration biomarkers are consistent with a neurodegenerative process that begins in the striatal terminals before progressing toward the cell bodies in the SNc.
Single-cell somatic copy number variants in brain using different amplification methods and reference genomes
By Emma Sherrell onThis study demonstrates that the authors' semi-automated protocol is suitable for shotgun metagenomic analysis, by significantly producing higher DNA fragment sizes while allowing for improved sample treatment logistics with reduced technical variability and without compromising the structure of the oral microbiome.
Testing AAVrg-hSyn-mCherry in the Putamen of Rhesus Macaques
By Emma Sherrell onViral Vector: Two rhesus macaque animals were tested for AAVrg-hSyn-mCherry in the putamen. The authors observed abundant mCherry-positive neurons in expected targets (i.e., motor cortical regions known to project to the putamen).
Immunofluorescent labeling of cultured cells
By savannah onThis protocol describes the process of Paraformaldehyde fixation and immunofluorescent-labeling of cultured cells.
Midbrain viral injections for striatal fiber photometry in mice
By savannah onWhile this protocol is focused on injections of AAV viruses into the midbrain, it can be easily modified to inject into other brain regions or other viruses.
pCMV-pOTC-GFP
By savannah onExpression of GFP fused with pOTC, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase.