3D-correlative FIB-milling and Cryo-ET of Autophagic structures in Yeast Cells

Description

This protocol describes how to plunge-freeze yeast on EM grids and how to target autophagic structures by combining cryo confocal fluorescence data to FIB-milling and tomogram acquisition.

Identifier (DOI)

10.17504/protocols.io.b79wrr7e

Tags

Autophagy
Cryo-ET

Team

Team Harper

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

Cristina Capitanio, MSc

Key Personnel: Team Harper,

Max Planck Institute of Biochemistry
Anna Bieber, MSc

Key Personnel: Team Harper,

Max Planck Institute of Biochemistry
Philipp S. Erdmann

External Collaborator
Brenda Schulman, PhD

Co-PI (Core Leadership): Team Harper,

Max Planck Institute of Biochemistry
Florian Wilfling, PhD

Collaborating PI: Team Harper, Team Hurley,

Max Planck Institute of Biophysics
Wolfgang Baumeister

External Collaborator

3D-correlative FIB-milling and Cryo-ET of Autophagic structures in Yeast Cells

Output Details

Meet the Authors

Cristina Capitanio, MSc

Anna Bieber, MSc

Philipp S. Erdmann

Brenda Schulman, PhD

Florian Wilfling, PhD

Wolfgang Baumeister

Related Research

pCAG-MBP-ATG9-V836A

Analysis of ER Flux in Cultured Induced Neurons using Keima ER reporters

Characterizing spatial and temporal properties of ER-phagy receptors