Quick guide to use paceTOMO for cryo-ET data collection from Titan Krios

View Published Protocol

Output Details

Description
This quick guide provides key minimal steps for preparing the Titan/SerialEM for the tomogram data collection on lamella or in vitro specimens with a K3 camera. paceTOMO routine is also included for a typical tomogram data collection session. Please note that this is not an exhaustive guide, but summarises the order of key steps.
Identifier (DOI)
10.17504/protocols.io.6qpvr3442vmk/v1
Team
Team Reck-Peterson
Program
Collaborative Research Network
Theme
PD Functional Genomics

Related Research

Gene expression profile at single cell levels of the cells from the controls and tau P251L Drosophila brain

Tau protein's role in Alzheimer's disease was studied by introducing a human tau mutation into Drosophila using CRISPR. Single-cell RNA sequencing helped identify genes and pathways involved in tauopathy.

View Dataset

scAlleleExpression: R package for downstream analysis of allele specific expression single cell data

An R package for investigating Cell Type specific regulatory genetics using single cell or nuclei allele specific expression data. It loads ASE pipeline output, performs ASE analysis, and works with various ASE pipelines.

View Code

scASE_py: Python pacakge for loading single cell allele specific data

Python package for single cell allele-specific expression (ASE) output manipulation available at https://github.com/seanken/ASE_pipeline. R package for downstream analysis at https://github.com/seanken/scAlleleExpression.

View Code
View More Outputs
Aligning Science Across Parkinson's
Powered by  GDPR Cookie Compliance
Privacy Overview

This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.