Western blot protocol for detecting ATP10B in mouse/rat brain
By onProtocol for detection of ATP10B in rat and mouse brain tissue by Western blotting.
Unconventional secretion of α-synuclein mediated by palmitoylated DNAJC5 oligomers
By onThe secretion of endogenous α-syn mediated by DNAJC5 is found in a human neuroblastoma cell line, The authors propose that DNAJC5 forms a palmitoylated oligomer to accommodate and export α-syn.
Flow cytometry-based measurement of mitophagic flux
By onAssociated with preprint: https://www.biorxiv.org/content/10.1101/2022.11.02.514817v1
Impaired dopamine release in Parkinson’s disease
By onEvidence indicates that impaired dopamine release can result from disruption to a diverse range of Parkinson’s disease-associated genetic and molecular disturbances and can be considered a potential pathophysiological hallmark of Parkinson’s disease.
SpatialBrain.org – a platform to view integrated data from Wade-Martins Laboratory of Molecular Neurodegeneration
By onAn open repository to access data and results from spatial and translatome expression profiling of basal dopaminergic neurons in mice (OVX and aging). More information can be viewed in published article Kilfeather & Khoo, et al. (2023) "Single-cell spatial transcriptomic and translatomic profiling of dopaminergic neurons in health, aging, and disease" Spatial Transcriptomic Analyses: - Cell Type Markers - SN/VTA Markers in Dopaminergic Neurons - Cell Number Changes in Age TRAP Analyses: - Dopaminergic Markers - Dopaminergic Ageing - Alternative Splicing in Dopaminergic Neurons Coming soon: Integration with iPS-derived dopaminergic transcriptomic datasets.
Modelling human brain-wide pigmentation in rodents recapitulates age-related multisystem neurodegenerative deficits
By onAuthors describe an animal model of human-like neuromelanin pigmentation. The animals display age-related neuronal dysfunction and degeneration affecting numerous brain circuits and body tissues.
Python script for Golgi cytoscape analysis
By onCustom Python script used for the cytoscape network analysis reported in doi.org/10.1101/2022.11.22.517583 (Golgi-IP, a novel tool for multimodal analysis of Golgi molecular content).
POLCAM: Instant molecular orientation microscopy for the life sciences
By onPOLCAM is a simplified single-molecule orientation localization microscopy method, using a polarization camera, enabling fast easy implementation on fluorescence microscopes. Here, we apply it to alpha-synuclein fibrils.
BPK1520-LRRK2-G2019S-ng
By onPlasmid: This resource can be used to introduce LRRK2-G2019S mutation using prime editing, in PE3 approach.
A mono- and intralink filter (mi-filter) to reduce false identifications in cross-linking mass spectrometry data
By onCross-linking mass spectrometry (XL-MS) has become an indispensable tool for the emerging field of systems structural biology over the recent years. However, the confidence in individual protein–protein interactions (PPIs) depends on the correct assessment of individual inter-protein cross-links. In this article, we describe a mono- and intralink filter (mi-filter) that is applicable to any kind of cross-linking data and workflow. It stipulates that only proteins for which at least one monolink or intra-protein cross-link has been identified within a given data set are considered for an inter-protein cross-link and therefore participate in a PPI. We show that this simple and intuitive filter has a dramatic effect on different types of cross-linking data ranging from individual protein complexes over medium-complexity affinity enrichments to proteome-wide cell lysates and significantly reduces the number of false-positive identifications for inter-protein links in all these types of XL-MS data.
H9 ES AAVS1-NGN2 FAM134C/A/B-/-;TEX264-/-; CCPG1-/-; PiggyBac-Keima-REEP5
By onES cells were modified to produce iNeurons lacking ER-phagy receptor genes and expressing Keima-REEP5 reporter. CRISPR/Cas9 was used to introduce NEUROG2 construct in AAVS1 locus, and various genes were knocked out.
pCAG-MBP-Foldon-ATG9 (692-839)
By onPlasmid for expression of ATG9 C-terminal tail trimer in mammalian cells.
LC3 Lipidation assay for ATG3 Mutants
By onProtocol describing the procedure to perform LC3 lipidation reaction assay on liposomes (SUVs, Small Unilamellar Vesicles).
Synaptotagmin-1-dependent phasic axonal dopamine release is dispensable for basic motor behaviors in mice
By onIn Parkinson’s disease (PD), motor dysfunctions only become apparent after extensive loss of DA innervation. This resilience has been hypothesized to be due to the ability of many motor behaviors to be sustained through a diffuse basal tone of DA; but experimental evidence for this is limited. Here we show that conditional deletion of the calcium sensor synaptotagmin-1 (Syt1) in DA neurons (Syt1 cKODA mice) abrogates most activity-dependent axonal DA release in the striatum and mesencephalon, leaving somatodendritic (STD) DA release intact. Strikingly, Syt1 cKODA mice showed intact performance in multiple unconditioned DA-dependent motor tasks and even in a task evaluating conditioned motivation for food. Considering that basal extracellular DA levels in the striatum were unchanged, our findings suggest that activity-dependent DA release is dispensable for such tasks and that they can be sustained by a basal tone of extracellular DA. Taken together, our findings reveal the striking resilience of DA-dependent motor functions in the context of a near-abolition of phasic DA release, shedding new light on why extensive loss of DA innervation is required to reveal motor dysfunctions in PD.
Design and preparation of synthetic reference peptides for APP/Aβ TOMAHAQ proteomics, version 2
By onThis protocol describes the design and preparation of synthetic reference peptides for APP/Aβ TOMAHAQ proteomics.
Thawing of feeder-free hPSCs
By onThis protocol describes the procedure of thawing feeder-free human pluripotent stem cells (hPSCs) using mTeSR-plus or StemFlex.
The chaperone Clusterin in neurodegeneration−friend or foe?
By onThe authors review the diverse functions of Clusterin in the pathogenesis of neurodegenerative diseases, focusing on evidence that Clusterin may act either as a suppressor or enhancer of pathology.
