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Raw tomogram of a TauRD-YFP aggregate in a TauRD-YFP expressing HEK293T cells and primary mouse neurons.

By Itika Saha, PhD, Patricia Yuste Checa, PhD, Miguel Da Silva Padilha, Qiang Guo, Roman Körner, Hauke Holthusen, Victoria Anne Trinkaus, MSc, Irina Dudanova, Ruben Fernandez-Busnadiego, PhD, Wolfgang Baumeister, David W. Sanders, Saurabh Gautam, Franz-Ulrich Hartl, MD and Mark S. Hipp
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Output Details

Description
Raw tomogram of a TauRD-YFP aggregate in a TauRD-YFP expressing Hek293T cells. Accession numbers: HEK293T: https://www.ebi.ac.uk/emdb/EMD-13739 Primary mouse neurons: https://www.ebi.ac.uk/emdb/EMD-13740
Tags
  • Cryo-ET
  • Human
  • In Situ
  • Microscopy - electron
  • Mouse
  • Primary culture
  • Protein aggregates
Team
Team Harper
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

  • Itika Saha, PhD

    Key Personnel: Team Harper, 

    Max Planck Institute of Biochemistry

  • Patricia Yuste Checa, PhD

    Key Personnel: Team Harper, 

    Max Planck Institute of Biochemistry

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    Miguel Da Silva Padilha

    External Collaborator

  • User avatar fallback logo

    Qiang Guo

    External Collaborator

  • User avatar fallback logo

    Roman Körner

    External Collaborator

  • User avatar fallback logo

    Hauke Holthusen

    External Collaborator

  • Victoria Anne Trinkaus, MSc

    Key Personnel: Team Harper, 

    Max Planck Institute of Biochemistry

  • User avatar fallback logo

    Irina Dudanova

    External Collaborator

  • Ruben Fernandez-Busnadiego, PhD

    Co-PI (Core Leadership): Team Harper, 

    University of Gottingen

  • User avatar fallback logo

    Wolfgang Baumeister

    External Collaborator

  • User avatar fallback logo

    David W. Sanders

    External Collaborator

  • User avatar fallback logo

    Saurabh Gautam

    External Collaborator

  • Franz-Ulrich Hartl, MD

    Co-PI (Core Leadership): Team Harper, 

    Max Planck Institute of Biochemistry

  • User avatar fallback logo

    Mark S. Hipp

    External Collaborator

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Cryo-electron tomography of thinned synapses

Protocol for cryo-ET of thinned synapses in rat neuron cultures is detailed. Cryo-FIB milling creates thin samples for high-resolution imaging. Synapse targeting with and without cryo-FLM correlation is explained.

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Preparing primary sandwich hippocampal neuron cultures for cryo-electron tomography

Sandwich hippocampal neuron cultures have low cell density and few glial cells, making them ideal for targeting neurons for cryo-electron tomography.

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Aligning Science Across Parkinson's
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