HyDrop Bead Generation & PCR Barcoding V.2
Output Details
Description
This protocol details hydrogel bead generation and barcoding for HyDrop v2. The barcodes are build by split-pool and ligation strategy (3x96). Here, we will create an emulsion of acrylamide monomers in a carrier oil containing TEMED. The monomer droplets will polymerise and form hydrogel beads. Ideally, you have a bead stock of around 3 mL of beads before you barcode, but 2 mL can work as well. It is best to produce the beads in one single run, from a single bead mixture to prevent disparities in sizes and/or primer concentration within the bead from occurring.
Identifier (DOI)
10.17504/protocols.io.8epv5n11dv1b/v2
