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Mitochondrial Antigen Presentation in RAW macrophages

By Ahmed Fahmy, PhD, Camberly Hernandez, MSc
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Output Details

Description
This protocol details methods for 3-day Mitochondrial Antigen Presentation Assay in a murine macrophage cell line (RAW) that expresses a glycoprotein B (gB) of herpes simplex virus 1 (HSV1) targeted to the mitochondrial matrix (mito-gB). A gB-specific CD8+ T cell hybridoma recognizing the gB498–505peptide loaded on MHC class I molecules is also used to monitor antigen presentation through a beta-galactosidase assay kit.
Tags
  • Antigen
  • Macrophage
  • Mitochondrial
Team
Team Desjardins
Program
Collaborative Research Network
Theme
Neuro-Immune Interactions

Meet the Authors

  • Ahmed Fahmy, PhD

    Key Personnel: Team Desjardins, 

    University of Montreal

  • User avatar fallback logo

    Camberly Hernandez, MSc

    Key Personnel: Team Desjardins, 

    University of Montreal

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Aligning Science Across Parkinson's
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