ATG3 construct cloning
By onProtocol describing the cloning of ATG3 construct for production of recombinant (GFP tagged) ATG3 variants.
Neuromelanin-positive Neuron Density in Substantia Nigra Image Analysis
By onThe protocol covers the steps to measure neuromelanin-positive neuron density in substantia nigra using image analysis tools, including NZConnect (Hamamatsu), a web-based whole-slide image (WSI) viewer, Cellpose, and QuPath.
Preparation of Acute Brain Slices
By onThis protocol describes the steps for preparing acute brain slices.
Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK
By onMuch of the biology surrounding Parkin function has taken place in artificial cell systems. The authors used human neurons to identify and validate 22 protein targets of Parkin, providing a functional Parkin landscape in neuronal cells.
Chemical degradation and determination of pheomelanin and eumelanin markers
By onThis is protocol involving chemical oxidation and reduction methods followed by high performance liquid chromatography (HPLC) detection of markers for pheomelanin and eumelanin.
Lysosome proteolysis analysis with DQ-BSA
By onProtocol to analyze lysosomal proteolysis in astrocytes generated from iPSCs.
Immunohistochemistry of Human Brain Striatum: ciliation of cholinergic neurons and astrocytes
By onThe authors present a method to identify the same brain region in human postmortem tissue to evaluate ciliary status. The striatum is identified morphologically and also by immunofluorescence microscopy using anti-DARPP-32 antibodies.
GFP-YIPF3 Immunoprecipitation
By onThe protocol outlines the immunoprecipitation procedure for GFP-YIPF3 protein.
Analysis of glycosphingolipids from human cerebrospinal fluid
By onThe method uses the fluorescent compound anthranilic acid (2-AA) to label oligosaccharides prior to analysis using normal-phase high-performance liquid chromatography. The labeling procedure is rapid, selective, and easy to perform.
Gene editing of YIPF4, FIP200, and ATG7 in HEK293 cells
By onProtocol outlines creation of YIPF4, FIP200, and ATG7 knockout cell lines in HEK293 cells via CRISPR-Cas9 method.
pFastBac_Dual_GST-TEV-EGFP-TBK1
By onPlasmid: Plasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631
From structure to ætiology: a new window on the biology of leucine-rich repeat kinase 2 and Parkinson’s disease
By onThis review summarizes LRRK2 structure both in a historical and current context, highlighting new insights into the structure of LRRK2 and complexes it forms.
pU6-pegRNA-LRRK2-G2019S-3d
By onIt can be used to introduce LRRK2-G2019S mutation using prime editing.
pET30a(+)-PE2
By onPlasmid: This resource can be used to express and purify PE2 protein for RNP delivery.
