pCMV-pOTC-3H-GFP
By savannah onExpression of GFP fused with pOTC-3H, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase and a thermostable three helix-bundle.
Tail suspension test to assess depression/anxiety behavior in parkinsonian mice
By savannah onThe Tail Suspension Test is a mouse behavioral paradigm measuring behavioral despair or “depression-like” behavior and learned helplessness.
Transcardiac perfusion of mouse for brain tissue
By savannah onTranscardiac perfusion is a method used for clearing blood and preserving the mouse brain for immunostaining.
Surgical procedures for simultaneous optogenetic manipulation in the SNc and acute fiber photometry in the dorsal striatum
By savannah onThis protocol describes the three surgical procedures required for simultaneous optogenetic stimulation in the Substantia Nigra pars compacta (SNc) and acute fiber photometry in the dorsal striatum.
Striatal cannula window implantation
By savannah onThis protocol describes how to implant a cannula window over the dorsolateral striatum in mice to enable two-photon imaging of dopamine axons in the dorsal striatum.
Stereotactic viral injection into subthalamic nucleus in mice
By savannah onThis protocol describes the surgical procedure of injecting AAV viruses into the subthalamic nucleus of a mouse. It contains information regarding the stereotactic setup, surgery preparation, anesthesia, craniotomy, injection, and recovery. This protocol has been adapted from Maite Azcorra's protocol for midbrain viral injections.
Sectioning of mouse brain by microtome
By savannah onThis protocol describes how to use the microtome to prepare and slice mouse brain sections for Immunohistochemistry.
Sectioning of mouse brain by cryostat
By savannah onThis protocol describes how to use the cryostat to prepare and slice mouse brain sections for Immunohistochemistry.
Rotarod Test to assess motor coordination in a mouse parkinsonian model
By savannah onThis protocol evaluates motor coordination in the rotarod assay in parkinsonian mice.
Pole Test to assess motor coordination in parkinsonian mice
By savannah onThe pole test evaluates the motor coordination ability of a mouse by having them grasp a pole and then descend to its home cage.
Open Field Test to assess motor coordination in a mouse parkinsonian model
By savannah onThe Open Field task is a simple sensorimotor test used to determine general activity levels, gross locomotor activity, and exploration habits in rodent models of CNS disorders.
Isolation and processing of embryonic and postnatal brains
By savannah onThis protocol describes: -Harvesting embryos from females from embryonic day 10 to embryonic day 18 -Brain isolation of embryonic (e10-e18) and postnatal brains (p0-p30) -Processing and freezing of embryonic and postnatal brains.
