Assay for Dual Rab GTPase binding to the LRRK2 Armadillo Domain

Output Details

The LRRK2 Armadillo domain contains multiple Rab GTPase binding sites. To show that the sites can be occupied simultaneously, we use this assay. The idea is to immobilize Rab8A, bind Armadillo domain, and test if phosphoRab10 can bind to Rab8A-immobilized Armadillo domain.

Tags
  • pRabs
  • Rab GTPases

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