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Assay for PhosphoRab activation of LRRK2 Kinase
Output Details
Description
MST kinase phosphorylates Rab proteins at the same site as LRRK2 and has been used to phosphorylate Rab8A and Rab10 quantitatively. This protocol includes a method to produce phosphoRab8A protein and remove as much contaminating MST3 as possible, to enable use of the phosphoRab to test subsequent activation of LRRK2 kinase.
Identifier (DOI)
10.17504/protocols.io.6qpvr4o8zgmk/v1