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Generation of human-induced pluripotent-stem-cell-derived cortical neurons for high-throughput imaging of neurite morphology and neuron maturation

By Gautam Wali, PhD, Kwaku Dad Abu-Bonsrah, PhD, yan li, Deniz Kirik, MD, PhD, Clare Parish, PhD and Carolyn Sue, PhD
Published June 8, 2023
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Output Details

Published June 8, 2023

Description
A STAR protocol that describes how to differentiate cryopreserved human cortical neuronal progenitors into mature cortical neurons for high-throughput imaging analysis
Identifier (DOI)
10.1016/j.xpro.2023.102325
Tags
  • 2D cultures
  • Analysis
  • Imaging
  • Original Research
Team
Team Kirik
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

  • Gautam Wali, PhD

    Key Personnel: Team Kirik, 

    University of Sydney

  • Kwaku Dad Abu-Bonsrah, PhD

    Key Personnel: Team Kirik, 

    Florey Institute of Neuroscience and Mental Health

  • User avatar fallback logo

    yan li

    External Collaborator

  • Deniz Kirik, MD, PhD

    Lead PI (Core Leadership): Team Kirik, 

    Lund University

  • Clare Parish, PhD

    Co-PI (Core Leadership): Team Kirik, 

    Florey Institute of Neuroscience and Mental Health

  • Carolyn Sue, PhD

    Collaborating PI: Team Kirik, 

    Neuroscience Research Australia

Related Research

Datasets and Key Resources Table used in Do, Quyen B. et al. (2023) “Early striatal hyperexcitability in an in vitro human striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation”

The files found here are associated to Do, Q. et al (2023) study and contain: (a) all source and supplementary data, (b) Key Resources Table, and (c) list of primers.

View Dataset

Early deficits in an in vitro striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation

The results highlight the unique utility of modeling striatal neurons in a modular and highly physiological circuit, which is essential to reveal mechanistic insights of the loss of electrical functional integrity in the striata of GBA1 PD patients.

View Article

STC-1 cell culture

Cell culture of STC-1 mouse enteroendocrine cells.

View Protocol
View More Outputs
Aligning Science Across Parkinson's
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