Structural pathway for PI3-kinase regulation by VPS15 in autophagy
By onCryo-EM analysis reveals activation pathway of PI3KC3-C1 in atomistic detail.
Next-generation sequencing of AAV.CAP-Mac enrichment from Chuapoco et al. (2023)
By onDataset of next-generation sequencing of enrichment of AAV.CAP-Mac in various tissues from the publication: Chuapoco, M.R., Flytzanis, N.C., Goeden, N. et al. Adeno-associated viral vectors for functional intravenous gene transfer throughout the non-human primate brain. Nat. Nanotechnol. (2023). https://doi.org/10.1038/s41565-023-01419-x
Multiple genome alignment in the telomere-to-telomere assembly era
By onThis review provides an overview of the algorithmic template that most multiple genome alignment methods follow.
Cell Culture
By onThis protocol describes routine cell culture maintenance as described in Adriaenssens & Nguyen et al., 2023 (BioRxiv)
Plasmid Construction and Gibson Cloning
By onThis protocol describes plasmid construction as described in Adriaenssens & Nguyen et al., 2023 (BioRxiv)
Phosphoglycerate kinase is a central leverage point in Parkinson’s disease–driven neuronal metabolic deficits
By onStudy shows increasing PGK1 gene expression enhances neuronal ATP production, protecting against synaptic dysfunction in Parkinson's disease. Boosting bioenergetics with PGK1 may be a promising therapeutic approach for PD.
Jensen_etal_2022_molecular_dynamics_simulation_data_2
By onFull molecular dynamics simulation data set accompanying Jensen et al, 2022, containing (i) representative coordinates and measurements described therein, (ii) simulation trajectories, and (iii) GROMACS input files.
A topographical atlas of α-synuclein dosage and cell type-specific expression in adult mouse brain and peripheral organs
By onThis atlas provides much-needed insight into the cellular topography of αSyn, and provides a quantitative map to test assumptions about the role of αSyn in network vulnerability in PD and other αSynucleinopathies.
Salivary collection participant instructions using OMNIgene ORAL OM 501 device
By onSalivary collection participant instructions using OMNIgene ORAL OM 501 device
R scripts for analysis of publication “Sex distribution of GBA1 Variants Carriers with Dementia with Lewy Bodies and Parkinson Disease”
By onR scripts for analysis of publication "Sex distribution of GBA1 variant carriers with dementia with Lewy Bodies and Parkinson's disease."
Sex Distribution of GBA1 Variants Carriers with Dementia with Lewy Bodies and Parkinson’s Disease
By onSex Distribution of GBA1 Variants Carriers with Dementia with Lewy Bodies and Parkinson's Disease
Biochemical consequences of glucocerebrosidase 1 mutations in Parkinson’s disease
By onPerspective on the biochemical consequences of glucocerebrosidase 1 mutations in Parkinson’s disease.
Primary data associated with the manuscript 10.1073/pnas.2315171120 (“Localization of PPM1H phosphatase tunes Parkinson’s disease-linked LRRK2 kinase-mediated Rab GTPase phosphorylation and ciliogenesis”)
By onPrimary data associated with the manuscript 10.1073/pnas.2315171120 ("Localization of PPM1H phosphatase tunes Parkinson’s disease-linked LRRK2 kinase-mediated Rab GTPase phosphorylation and ciliogenesis").
Transfection by electroporation of GFP-LRRK2 and Immunofluorescent imaging of MEFs VPS35 (D620N) mutants stably expressing LysoTag
By onThe authors investigated the colocalization of GFP-LRRK2 with a lysosomal localized TMEM192-3xHA.
Systems-level analyses dissociate genetic regulators of reactive oxygen species and energy production
By onRespiratory chain dysfunction affects ATP and ROS levels. Knockdown of genes in specific respiratory complexes increases ROS, while metabolic conditions have little impact on ROS. Ether lipids play a role in regulating ROS independently of ATP.
Reduced striatal M4-cholinergic signaling following dopamine loss contributes to parkinsonian and l-DOPA–induced dyskinetic behaviors
By onImbalances in dopamine and acetylcholine affect motor function in Parkinson's disease. Contrary to conventional theories, reduced cholinergic transmission at M4 receptors in dopamine-depleted mice alleviated motor deficits and dyskinetic behavior.
Adaptor protein-3 produces synaptic vesicles that release phasic dopamine
By onThe burst firing of midbrain dopamine neurons releases a phasic dopamine signal that mediates reinforcement learning. At many synapses, however, high firing rates deplete synaptic vesicles (SVs), resulting in synaptic depression that limits release. What accounts for the increased release of dopamine by stimulation at high frequency? We find that adaptor protein-3 (AP-3) and its coat protein VPS41 promote axonal dopamine release by targeting vesicular monoamine transporter VMAT2 to the axon rather than dendrites. AP-3 and VPS41 also produce SVs that respond preferentially to high-frequency stimulation, independent of their role in axonal polarity. In addition, conditional inactivation of VPS41 in dopamine neurons impairs reinforcement learning, and this involves a defect in the frequency dependence of release rather than the amount of dopamine released. Thus, AP-3 and VPS41 promote the axonal polarity of dopamine release but enable learning by producing a distinct population of SVs tuned specifically to high firing frequency that confers the phasic release of dopamine.
iATPSnFR2: A high-dynamic-range fluorescent sensor for monitoring intracellular ATP
By onImproved iATPSnFR2 sensor offers real-time ATP level monitoring in cells. Enhanced dynamic range, high specificity, and subcellular targeting capabilities enable detailed metabolic analysis in different cellular regions.
Lentiviral vector plasmid for overexpression of ATP10B (R153X)
By onLentiviral vector plasmid for overexpression of ATP10B (R153X) validated for use in cell culture
Lentiviral vector plasmid for overexpression of ATP10B (V748L)
By onLentiviral vector plasmid for overexpression of ATP10B (V748L) validated for use in cell culture
