Nucleofection of hPSCs

By Frank Soldner, MD, Hanqin Li, PhD, Steven Poser, PhD, Oriol Busquets Figueras, PhD and Dirk Hockemeyer, PhD
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Output Details

Description

This protocol describes the standard procedure for the delivery of plasmids, mRNA or ribonucleoprotein (RNP) into human pluripotent stem cells (hPSCs) using nucleofection.

Identifier (DOI)
10.17504/protocols.io.b4pcqviw
Tags
  • Nucleofection
Team
Team Rio
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

  • Frank Soldner, MD

    Co-PI (Core Leadership): Team Rio, 

    Albert Einstein College of Medicine

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    Hanqin Li, PhD

    Key Personnel: Team Rio, 

    University of California, Berkeley

  • Steven Poser, PhD

    Key Personnel: Team Rio, 

    University of Washington

  • Oriol Busquets Figueras, PhD

    Key Personnel: Team Rio, 

    Albert Einstein College of Medicine

  • Dirk Hockemeyer, PhD

    Co-PI (Core Leadership): Team Rio, 

    University of California, Berkeley

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