Nucleofection (Amaxa) and electroporation (Biorad) of hPSCs

Description

This collection describes the standard procedure for the delivery of plasmids, mRNA or ribonucleoprotein (RNP) into human pluripotent stem cells (hPSCs) using nucleofection or electroporation.

Identifier (DOI)

10.17504/protocols.io.e6nvwkkewvmk/v2

Tags

Cell culture
Electroporation
FACS
hPSCs (Human pluripotent stem cells)
iPSCs (Induced pluripotent stem cells)
Nucleofection

Discovery Team

Team Rio

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

Oriol Busquets Figueras

External Collaborator
Hanqin Li

External Collaborator
Steven Poser

External Collaborator
Frank Soldner, MD

Co-PI (Core Leadership): Team Rio,

Albert Einstein College of Medicine
Dirk Hockemeyer, PhD

Co-PI (Core Leadership): Team Rio,

University of California, Berkeley

Nucleofection (Amaxa) and electroporation (Biorad) of hPSCs

Output Details

Meet the Authors

Oriol Busquets Figueras

Hanqin Li

Steven Poser

Frank Soldner, MD

Dirk Hockemeyer, PhD

Related Research

Feeder-free culturing of hPSCs

Proteomic analysis of iNeurons lacking ER-phagy receptors

Thawing, Passaging and Freezing of hPSCs on MEFs