Fluorescent Immunolabelling for Alpha-Synuclein in neuronal primary culture (Testing PFF toxicity)

Description

This protocol is designed to perform fluorescent immunolabelling on neuronal primary culture after PFF incubation. The labelling of phosphorylated Alpha-synuclein is considered as a marker of the PFF toxicity and should be performed to test the PFF before in vivo injection. See protocol for PFF preparation https://www.protocols.io/view/production-of-alpha-synuclein-preformed-fibrils-pf-b39rqr56 and protocol for neuronal primary culture https://www.protocols.io/view/primary-culture-cortical-hippocampal-neurons-e15-1-cfiztkf6

Identifier (DOI)

10.17504/protocols.io.kxygxzr6wv8j/v1

Tags

Activity Assay
Immunofluorescence
Immunohistochemistry

Team

Team Kaplitt

Program

Collaborative Research Network

Theme

Circuitry and Brain-Body Interactions

Meet the Authors

Sabina Marciano

Key Personnel: Team Kaplitt,

Weill Cornell Medicine
Rong Chen, MSc

Key Personnel: Team Kaplitt,

Johns Hopkins University
Ted Dawson, MD, PhD

Co-PI (Core Leadership): Team Kaplitt,

Johns Hopkins Medicine
Roberta Marongiu, PhD

Co-PI (Core Leadership): Team Kaplitt,

Weill Cornell Medicine

Fluorescent Immunolabelling for Alpha-Synuclein in neuronal primary culture (Testing PFF toxicity)

Output Details

Meet the Authors

Sabina Marciano

Rong Chen, MSc

Ted Dawson, MD, PhD

Roberta Marongiu, PhD

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