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Fluorescent Immunolabelling for Alpha-Synuclein in neuronal primary culture (Testing PFF toxicity)
Output Details
Description
This protocol is designed to perform fluorescent immunolabelling on neuronal primary culture after PFF incubation. The labelling of phosphorylated Alpha-synuclein is considered as a marker of the PFF toxicity and should be performed to test the PFF before in vivo injection. See protocol for PFF preparation https://www.protocols.io/view/production-of-alpha-synuclein-preformed-fibrils-pf-b39rqr56 and protocol for neuronal primary culture https://www.protocols.io/view/primary-culture-cortical-hippocampal-neurons-e15-1-cfiztkf6
Identifier (DOI)
10.17504/protocols.io.kxygxzr6wv8j/v1