Assessing enrichment of proteins in the mitochondrial fraction in HEK cells

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Description

This is a method for measuring protein enrichment on mitochondria in various conditions. In the resulting Western blot, one can assess the level of contamination of other organelles in the enrichment prep.

Identifier (DOI)

10.17504/protocols.io.bxrnpm5e

Tags

Expression
Mitochondria
Proteins
Western Blot

Team

Team Hurley

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

Olivia Harding, BSc

Key Personnel: Team Hurley,

University of Pennsylvania

Assessing enrichment of proteins in the mitochondrial fraction in HEK cells

Output Details

Meet the Authors

Olivia Harding, BSc

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