Assessing autophagy using the HaloTag-LC3B cleavage assay

By Marvin Skulsuppaisarn
View Published Protocol

Output Details

Description
Assay to detect HaloTag-LC3B during starvation autophagy based on  https://doi.org/10.7554/eLife.78923.
Identifier (DOI)
10.17504/protocols.io.e6nvwdo9zlmk/v1
Tags
  • Autophagy
  • LC3
  • Other
Team
Team Hurley
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

  • User avatar fallback logo

    Marvin Skulsuppaisarn

Related Research

Three-step docking by WIPI2, ATG16L1 and ATG3 delivers LC3 to the phagophore

In this manuscript, in a near-complete pathway from initial membrane recruitment to LC3 lipidation reaction, we show how a three-step targeting mechanism of the ATG12-ATG5-ATG16L1 machinery ensures a high level of regulatory control on autophagy.

View Article

Structural pathway for PI3-kinase regulation by VPS15 in autophagy

Cryo-EM analysis reveals activation pathway of PI3KC3-C1 in atomistic detail.

View Article

A lipid transfer-dependent feedback loop activates ATG9A compartments in autophagy initiation

Phagophore formation requires ATG9, ATG2 and PI3KC3-C1. Authors show that ATG2 is crucial for PI3P appearance on ATG9A compartments with little PI, suggesting a lipid transfer-driven feedback loop supporting lipid transfer and ATG8 lipidation.

View Article
View More Outputs
Aligning Science Across Parkinson's
Powered by  GDPR Cookie Compliance
Privacy Overview

This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.