Co-immunoprecipitation protocol to study LRRK2 binding to Rab12 in a cell-based assay

By Francesca Tonelli, PhD
View Published Protocol

Output Details

Description
This protocol describes the immunoprecipitation (IP) of FLAG-tagged LRRK2 from whole cell lysates to assess its interaction with Rab12. This method can be used to screen the impact that LRRK2 mutations have on its binding to Rab12 in cells, as well as the effect of any compound or cell treatment on LRRK2 interaction with Rab12.
Identifier (DOI)
10.17504/protocols.io.n92ldmbbnl5b/v1
Tags
  • Immunoprecipitation
  • LRRK2
  • Rab GTPases
Team
Team Alessi
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

  • Francesca Tonelli, PhD

    Key Personnel: Team Alessi, 

    University of Dundee

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This protocol describes the immunoprecipitation (IP) of FLAG-tagged LRRK2 from whole cell lysates to assess its interaction with Rab12. This method can be used to screen the impact that LRRK2 mutations have on its binding to Rab12 in cells, as well…

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